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Muscarinic acetylcholine receptor M1 mediates prostate cancer cell migration and invasion through hedgehog signaling.
- Source :
-
Asian journal of andrology [Asian J Androl] 2018 Nov-Dec; Vol. 20 (6), pp. 608-614. - Publication Year :
- 2018
-
Abstract
- The autonomic nervous system contributes to prostate cancer proliferation and metastasis. However, the exact molecular mechanism remains unclear. In this study, muscarinic acetylcholine receptor M1 (CHRM1) expression was measured via immunohistochemical analysis in human prostate cancer tissue array slides. PC-3, LNCaP, and A549 cells were treated with pirenzepine or carbachol, and the cell migration and invasion abilities were evaluated. Western blotting and quantitative real-time PCR were performed to measure GLI family zinc finger 1 (GLI1), patched 1 (PTCH1), and sonic hedgehog (SHH) expression levels. High expression of CHRM1 was found in early-stage human prostate cancer tissues. In addition, the selective CHRM1 antagonist pirenzepine inhibited PC-3, LNCaP, and A549 cell migration and invasion, but the agonist carbachol promoted the migration and invasion of these three cell lines. Muscarinic signaling can be relayed by hedgehog signaling. These data show that CHRM1 is involved in the regulation of prostate cancer migration and invasion through the hedgehog signaling pathway.<br />Competing Interests: None
- Subjects :
- Carbachol pharmacology
Cell Movement genetics
Cell Proliferation
Humans
Male
Muscarinic Agonists pharmacology
Muscarinic Antagonists pharmacology
Patched-1 Receptor genetics
Pirenzepine pharmacology
Prostatic Neoplasms genetics
Receptor, Muscarinic M1 antagonists & inhibitors
Zinc Finger Protein GLI1 genetics
Hedgehog Proteins genetics
Prostatic Neoplasms pathology
Receptor, Muscarinic M1 genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1745-7262
- Volume :
- 20
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Asian journal of andrology
- Publication Type :
- Academic Journal
- Accession number :
- 30027929
- Full Text :
- https://doi.org/10.4103/aja.aja_55_18