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GPCR-SAS: A web application for statistical analyses on G protein-coupled receptors sequences.

Authors :
Gómez Tamayo JC
Olivella M
Ríos S
Hoogstraat M
Gonzalez A
Mayol E
Deupi X
Campillo M
Cordomí A
Source :
PloS one [PLoS One] 2018 Jul 25; Vol. 13 (7), pp. e0199843. Date of Electronic Publication: 2018 Jul 25 (Print Publication: 2018).
Publication Year :
2018

Abstract

G protein-coupled receptors (GPCRs) are one of the largest protein families in mammals. They mediate signal transduction across cell membranes and are important targets for the pharmaceutical industry. The G Protein-Coupled Receptors-Sequence Analysis and Statistics (GPCR-SAS) web application provides a set of tools to perform comparative analysis of sequence positions between receptors, based on a curated structural-informed multiple sequence alignment. The analysis tools include: (i) percentage of occurrence of an amino acid or motif and entropy at a position or range of positions, (ii) covariance of two positions, (iii) correlation between two amino acids in two positions (or two sequence motifs in two ranges of positions), and (iv) snake-plot representation for a specific receptor or for the consensus sequence of a group of selected receptors. The analysis of conservation of residues and motifs across transmembrane (TM) segments may guide the design of more selective ligands or help to rationalize activation mechanisms, among others. As an example, here we analyze the amino acids of the "transmission switch", that initiates receptor activation following ligand binding. The tool is freely accessible at http://lmc.uab.cat/gpcrsas/.<br />Competing Interests: The authors have declared that no competing interests exist.

Details

Language :
English
ISSN :
1932-6203
Volume :
13
Issue :
7
Database :
MEDLINE
Journal :
PloS one
Publication Type :
Academic Journal
Accession number :
30044824
Full Text :
https://doi.org/10.1371/journal.pone.0199843