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Bienzyme Magnetic Nanobiocatalyst with Fe 3+ -Tannic Acid Film for One-Pot Starch Hydrolysis.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2018 Aug 22; Vol. 66 (33), pp. 8753-8760. Date of Electronic Publication: 2018 Aug 09. - Publication Year :
- 2018
-
Abstract
- In this study, a novel co-immobilization biocatalyst for one-pot starch hydrolysis was prepared through shielding enzymes on the Fe <subscript>3</subscript> O <subscript>4</subscript> /SiO <subscript>2</subscript> core-shell nanospheres by a Fe <superscript>3+</superscript> -tannic acid (TA) film. In brief, α-amylase and glucoamylase were covalently immobilized on amino-modified Fe <subscript>3</subscript> O <subscript>4</subscript> /SiO <subscript>2</subscript> core-shell nanospheres using glutarldehyde as a linker. Then, a Fe <superscript>3+</superscript> -TA protective film was formed through the self-assembly of the Fe <superscript>3+</superscript> and TA coordination complex (Fe <superscript>3+</superscript> -TA@Fe <subscript>3</subscript> O <subscript>4</subscript> /SiO <subscript>2</subscript> -enzymes). The film acts a "coating" to prevent the enzyme from denaturation and detachment, thus significantly improving its structural and operational stability. Furthermore, the immobilization efficiency reached 90%, and the maximum activity recovery of α-amylase and glucoamylase was 87 and 85%, respectively. More importantly, the bienzyme magnetic nanobiocatalyst with Fe <superscript>3+</superscript> -TA film could be simply recovered by a magnet. The Fe <superscript>3+</superscript> -TA@Fe <subscript>3</subscript> O <subscript>4</subscript> /SiO <subscript>2</subscript> -enzymes kept 55% of the original activity after reuse for 9 cycles, indicating outstanding reusability. However, the bienzyme magnetic nanobiocatalyst without Fe <superscript>3+</superscript> -TA film maintained 28% of the initial activity.
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 66
- Issue :
- 33
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 30052438
- Full Text :
- https://doi.org/10.1021/acs.jafc.8b02097