Back to Search
Start Over
A C 2 H 2 zinc-finger protein OsZFP213 interacts with OsMAPK3 to enhance salt tolerance in rice.
- Source :
-
Journal of plant physiology [J Plant Physiol] 2018 Oct; Vol. 229, pp. 100-110. Date of Electronic Publication: 2018 Jul 18. - Publication Year :
- 2018
-
Abstract
- Improvement of salt tolerance is one of the major targets in rice breeding. Here, we report that the zinc-finger protein (ZFP) OsZFP213 functions in enhancing salt tolerance in rice. OsZFP213 is localized in the nucleus and has transactivation activity. Transgenic rice overexpressing OsZFP213 showed enhanced salt tolerance compared with wild type and OsZFP213 RNAi plants. Furthermore, OsZFP213 overexpression plants showed higher transcription levels of antioxidant system genes and higher catalytic activity of scavenging enzymes of reactive oxygen, such as superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), and glutathione reductase (GR), and a lower level of ROS accumulation than that in wild type and OsZFP213 RNAi plants under salt treatment. Yeast two-hybrid, pull-down, and BiFC analysis showed that OsMAPK3 is a direct partner of OsZFP213, and this interaction enhanced the transactivation activity of OsZFP213. Taken together, these results suggest that OsZFP213 cooperates with OsMAPK3 in the regulation of rice salt stress tolerance by enhancing the ability of scavenging reactive oxygen.<br /> (Copyright © 2018. Published by Elsevier GmbH.)
- Subjects :
- Ascorbate Peroxidases metabolism
Catalase metabolism
Gene Expression Regulation, Plant drug effects
Glutathione Reductase metabolism
Mitogen-Activated Protein Kinase 3 metabolism
Oxidative Stress drug effects
Reactive Oxygen Species metabolism
Salt Tolerance
Sodium Chloride pharmacology
Superoxide Dismutase metabolism
Oryza drug effects
Oryza metabolism
Plant Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1618-1328
- Volume :
- 229
- Database :
- MEDLINE
- Journal :
- Journal of plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 30055519
- Full Text :
- https://doi.org/10.1016/j.jplph.2018.07.003