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Senescence and odontoblastic differentiation of dental pulp cells.
- Source :
-
Journal of cellular physiology [J Cell Physiol] 2018 Jan; Vol. 234 (1), pp. 849-859. Date of Electronic Publication: 2018 Aug 04. - Publication Year :
- 2018
-
Abstract
- Cellular senescence has been suggested to be involved in physiological changes of cytokine production. Previous studies showed that the concentration of tumor necrosis factor-α (TNF-α) is higher in the blood of aged people compared with that of young people. So far, the precise effects of TNF-α on the odontoblastic differentiation of pulp cells have been controversial. Therefore, we aimed to clarify how this cytokine affected pulp cells during aging. Human dental pulp cells (HDPCs) were cultured until reaching the plateau of their growth, and the cells were isolated at actively (young HDPCs; yHDPCs) or inactively (senescent HDPCs; sHDPCs) proliferating stages. sHDPCs expressed senescence-related molecules while yHDPCs did not. When these HDPCs were cultured in an odontoblast-inductive medium, both young and senescent cells showed mineralization, but mineralization in sHDPCs was lower compared with yHDPCs. However, the administration of TNF-α to this culture medium altered these responses: yHDPCs showed downregulated mineralization, while sHDPCs exhibited significantly increased mineralization. Furthermore, the expression of tumor necrosis factor receptor 1 (TNFR1), a receptor of TNF-α, was significantly upregulated in sHDPCs compared with yHDPCs. Downregulation of TNFR1 expression led to decreased mineralization of TNF-α-treated sHDPCs, whereas restored the reduction in TNF-α-treated yHDPCs. These results suggested that sHDPCs preserved the odontoblastic differentiation capacity and TNF-α promoted odontoblastic differentiation of HDPCs with the progress of their population doublings through increased expression of TNFR1. Thus, TNF-α might exert a different effect on the odontoblastic differentiation of HDPCs depending on their proliferating activity. In addition, the calcification of pulp chamber with age may be related with increased reactivity of pulp cells to TNF-α.<br /> (© 2018 Wiley Periodicals, Inc.)
- Subjects :
- Calcification, Physiologic genetics
Cell Differentiation drug effects
Cell Proliferation drug effects
Dental Pulp drug effects
Dental Pulp growth & development
Gene Knockdown Techniques
Humans
Odontoblasts drug effects
Receptors, Tumor Necrosis Factor, Type I genetics
Tumor Necrosis Factor-alpha genetics
Aging genetics
Dental Pulp cytology
Odontoblasts cytology
Tumor Necrosis Factor-alpha pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4652
- Volume :
- 234
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of cellular physiology
- Publication Type :
- Academic Journal
- Accession number :
- 30078208
- Full Text :
- https://doi.org/10.1002/jcp.26905