Back to Search Start Over

Heteromeric RNP Assembly at LINEs Controls Lineage-Specific RNA Processing.

Authors :
Attig J
Agostini F
Gooding C
Chakrabarti AM
Singh A
Haberman N
Zagalak JA
Emmett W
Smith CWJ
Luscombe NM
Ule J
Source :
Cell [Cell] 2018 Aug 23; Vol. 174 (5), pp. 1067-1081.e17. Date of Electronic Publication: 2018 Aug 02.
Publication Year :
2018

Abstract

Long mammalian introns make it challenging for the RNA processing machinery to identify exons accurately. We find that LINE-derived sequences (LINEs) contribute to this selection by recruiting dozens of RNA-binding proteins (RBPs) to introns. This includes MATR3, which promotes binding of PTBP1 to multivalent binding sites within LINEs. Both RBPs repress splicing and 3' end processing within and around LINEs. Notably, repressive RBPs preferentially bind to evolutionarily young LINEs, which are located far from exons. These RBPs insulate the LINEs and the surrounding intronic regions from RNA processing. Upon evolutionary divergence, changes in RNA motifs within LINEs lead to gradual loss of their insulation. Hence, older LINEs are located closer to exons, are a common source of tissue-specific exons, and increasingly bind to RBPs that enhance RNA processing. Thus, LINEs are hubs for the assembly of repressive RBPs and also contribute to the evolution of new, lineage-specific transcripts in mammals. VIDEO ABSTRACT.<br /> (Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1097-4172
Volume :
174
Issue :
5
Database :
MEDLINE
Journal :
Cell
Publication Type :
Academic Journal
Accession number :
30078707
Full Text :
https://doi.org/10.1016/j.cell.2018.07.001