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Heteromeric RNP Assembly at LINEs Controls Lineage-Specific RNA Processing.
- Source :
-
Cell [Cell] 2018 Aug 23; Vol. 174 (5), pp. 1067-1081.e17. Date of Electronic Publication: 2018 Aug 02. - Publication Year :
- 2018
-
Abstract
- Long mammalian introns make it challenging for the RNA processing machinery to identify exons accurately. We find that LINE-derived sequences (LINEs) contribute to this selection by recruiting dozens of RNA-binding proteins (RBPs) to introns. This includes MATR3, which promotes binding of PTBP1 to multivalent binding sites within LINEs. Both RBPs repress splicing and 3' end processing within and around LINEs. Notably, repressive RBPs preferentially bind to evolutionarily young LINEs, which are located far from exons. These RBPs insulate the LINEs and the surrounding intronic regions from RNA processing. Upon evolutionary divergence, changes in RNA motifs within LINEs lead to gradual loss of their insulation. Hence, older LINEs are located closer to exons, are a common source of tissue-specific exons, and increasingly bind to RBPs that enhance RNA processing. Thus, LINEs are hubs for the assembly of repressive RBPs and also contribute to the evolution of new, lineage-specific transcripts in mammals. VIDEO ABSTRACT.<br /> (Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Alternative Splicing
Animals
Binding Sites
Exons
HeLa Cells
Humans
Introns
Mice
Mutation
Nucleotide Motifs
Phylogeny
Protein Binding
Protein Interaction Mapping
RNA Splicing
Heterogeneous-Nuclear Ribonucleoproteins chemistry
Long Interspersed Nucleotide Elements
Nuclear Matrix-Associated Proteins chemistry
Polyadenylation
Polypyrimidine Tract-Binding Protein chemistry
RNA chemistry
RNA-Binding Proteins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4172
- Volume :
- 174
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Cell
- Publication Type :
- Academic Journal
- Accession number :
- 30078707
- Full Text :
- https://doi.org/10.1016/j.cell.2018.07.001