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Detection of identical T cell clones in peritumoral pleural effusion and pneumonitis lesions in a cancer patient during immune-checkpoint blockade.
- Source :
-
Oncotarget [Oncotarget] 2018 Jul 17; Vol. 9 (55), pp. 30587-30593. Date of Electronic Publication: 2018 Jul 17 (Print Publication: 2018). - Publication Year :
- 2018
-
Abstract
- Although immune-related adverse events (irAEs) of treatment with immune-checkpoint inhibitors may be due to cellular immunity mediated by T lymphocytes, their pathogenesis has remained unknown. Here we collected bronchoalveolar lavage fluid (BALF) from a cancer patient with nivolumab-induced pneumonitis and isolated mononuclear cells for next-generation sequencing of the complementarity-determining region of the T cell receptor (TCR) β chain. Mononuclear cells in peritumoral pleural effusion isolated from the patient were similarly analyzed, and the results obtained for the two specimens were compared. A substantial number of TCRβ clones in BALF were also identified among lymphocytes in the peritumoral pleural effusion. Such a correlation was not apparent between TCRβ clones in BALF and those in peripheral blood. Moreover, many tumor-associated clones with a read frequency of ≥0.10% were also present in BALF. Our data suggest that irAEs might be induced by drug-activated lymphocytes originating from tumor tissue. Deep sequencing will thus be indispensable for investigations of the immune-based pathogenesis of, and the development of optimal treatments for, irAEs.<br />Competing Interests: CONFLICTS OF INTEREST The authors declare no conflicts of interest.
Details
- Language :
- English
- ISSN :
- 1949-2553
- Volume :
- 9
- Issue :
- 55
- Database :
- MEDLINE
- Journal :
- Oncotarget
- Publication Type :
- Academic Journal
- Accession number :
- 30093971
- Full Text :
- https://doi.org/10.18632/oncotarget.25743