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Ultraviolet Irradiation of Skin Alters the Faecal Microbiome Independently of Vitamin D in Mice.
- Source :
-
Nutrients [Nutrients] 2018 Aug 11; Vol. 10 (8). Date of Electronic Publication: 2018 Aug 11. - Publication Year :
- 2018
-
Abstract
- Reduced sunlight exposure has been associated with an increased incidence of Crohn's disease and ulcerative colitis. The effect of ultraviolet radiation (UVR) on the faecal microbiome and susceptibility to colitis has not been explored. C57Bl/6 female mice were fed three different vitamin D-containing diets for 24 days before half of the mice in each group were UV-irradiated (1 kJ/m²) for each of four days, followed by twice-weekly irradiation of shaved dorsal skin for 35 days. Faecal DNA was extracted and high-throughput sequencing of the 16S RNA gene performed. UV irradiation of skin was associated with a significant change in the beta-diversity of faeces compared to nonirradiated mice, independently of vitamin D. Specifically, members of phylum Firmicutes, including Coprococcus , were enriched, whereas members of phylum Bacteroidetes, such as Bacteroidales, were depleted. Expression of colonic CYP27B1 increased by four-fold and IL1β decreased by five-fold, suggesting a UVR-induced anti-inflammatory effect. UV-irradiated mice, however, were not protected against colitis induced by dextran sodium sulfate (DSS), although distinct faecal microbiome differences were documented post-DSS between UV-irradiated and nonirradiated mice. Thus, skin exposure to UVR alters the faecal microbiome, and further investigations to explore the implications of this in health and disease are warranted.
- Subjects :
- Animal Feed
Animals
Bacteria classification
Colitis blood
Colitis chemically induced
Colitis pathology
Cytokines blood
Dextran Sulfate
Disease Models, Animal
Female
Inflammation Mediators blood
Mice, Inbred C57BL
Bacteria drug effects
Bacteria radiation effects
Cholecalciferol administration & dosage
Colitis microbiology
Feces microbiology
Gastrointestinal Microbiome drug effects
Gastrointestinal Microbiome radiation effects
Skin radiation effects
Ultraviolet Rays
Subjects
Details
- Language :
- English
- ISSN :
- 2072-6643
- Volume :
- 10
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Nutrients
- Publication Type :
- Academic Journal
- Accession number :
- 30103486
- Full Text :
- https://doi.org/10.3390/nu10081069