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Tisp40 deficiency limits renal inflammation and promotes tubular cell proliferation in renal ischemia reperfusion injury.
- Source :
-
Experimental cell research [Exp Cell Res] 2018 Oct 01; Vol. 371 (1), pp. 255-261. Date of Electronic Publication: 2018 Aug 17. - Publication Year :
- 2018
-
Abstract
- Renal ischemia reperfusion (IR) is a common cause of acute kidney injury (AKI), and no effective treatment is available to date. In our previous studies, we demonstrated that Tisp40 exacerbates tubular cell apoptosis and tubulointerstitial fibrosis after renal IR injury. However, the role of Tisp40 in renal inflammatory responses and tubular cell proliferation during renal IR injury remains unknown. In this study, Tisp40 knockout (KO) and wild-type (WT) mice were induced with or without renal IR injury. For renal IR, bilateral renal pedicels were exposed and clamped to induce 30 min of ischemia. After 48 h of reperfusion, the kidneys were collected for analyses. Results showed that Tisp40 deficiency attenuates neutrophil and macrophage infiltration after renal IR. Consistently, the protein levels of TNF-α and MCP-1 were markedly decreased, and the phosphorylation levels of IκBα and P65 were inhibited in Tisp40-deficient mice than in WT mice in renal IR injury. In addition, compared with WT mice, Tisp40 deficiency significantly increased the expression levels of proliferative cellular nuclear antigen and phosphorylated Erk1/2 after renal IR injury. In conclusion, Tisp40 deficiency limits renal inflammatory responses and promotes tubular cell proliferation in ischemic AKI.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cell Movement
Cell Proliferation
Chemokine CCL2 genetics
Chemokine CCL2 metabolism
Cyclic AMP Response Element-Binding Protein deficiency
Epithelial Cells cytology
Gene Expression Regulation
Inflammation
Kidney Tubules, Proximal pathology
Macrophages metabolism
Macrophages pathology
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Mitogen-Activated Protein Kinase 1 genetics
Mitogen-Activated Protein Kinase 1 metabolism
Mitogen-Activated Protein Kinase 3 genetics
Mitogen-Activated Protein Kinase 3 metabolism
NF-KappaB Inhibitor alpha genetics
NF-KappaB Inhibitor alpha metabolism
Nephritis, Interstitial metabolism
Nephritis, Interstitial pathology
Nephritis, Interstitial prevention & control
Neutrophils metabolism
Neutrophils pathology
Phosphorylation
Proliferating Cell Nuclear Antigen genetics
Proliferating Cell Nuclear Antigen metabolism
Reperfusion Injury metabolism
Reperfusion Injury pathology
Reperfusion Injury prevention & control
Signal Transduction
Transcription Factor RelA genetics
Transcription Factor RelA metabolism
Tumor Necrosis Factor-alpha genetics
Tumor Necrosis Factor-alpha metabolism
Cyclic AMP Response Element-Binding Protein genetics
Epithelial Cells metabolism
Kidney Tubules, Proximal metabolism
Nephritis, Interstitial genetics
Reperfusion Injury genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2422
- Volume :
- 371
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Experimental cell research
- Publication Type :
- Academic Journal
- Accession number :
- 30121191
- Full Text :
- https://doi.org/10.1016/j.yexcr.2018.08.019