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Overexpression of Gilz Protects Mice Against Lethal Septic Peritonitis.
- Source :
-
Shock (Augusta, Ga.) [Shock] 2019 Aug; Vol. 52 (2), pp. 208-214. - Publication Year :
- 2019
-
Abstract
- Sepsis in humans and experimental animals is characterized by an acute inflammatory response. glucocorticoids (GCs) are widely used for the treatment of many inflammatory disorders, yet their effectiveness in sepsis is debatable. One of the major anti-inflammatory proteins induced by GCs is glucocorticoid-induced leucine zipper (GILZ, coded by the TSC22D3 gene). We found that TSC22D3 mRNA expression is downregulated in white blood cells of human sepsis patients. Interestingly, transgenic GILZ-overexpressing mice (GILZ-tg) showed better survival rates in the cecal ligation and puncture (CLP) model of mouse sepsis. To our surprise, GILZ had only mild anti-inflammatory effects in this model, as the systemic proinflammatory response was not significantly reduced in GILZ-tg mice compared with control mice. During CLP, we observed reduced bacterial counts in blood of GILZ-tg mice compared with control mice. We found increased expression of Tsc22d3 mRNA specifically in peritoneal exudate cells in the CLP model, as well as increased capacity for bacterial phagocytosis of CD45 GILZ-tg cells compared with CD45 GILZ-wt cells. Hence, we believe that the protective effects of GILZ in the CLP model can be linked to a more efficient phagocytosis.
- Subjects :
- Animals
Cecum injuries
Humans
Interleukin-6 blood
Leukocyte Common Antigens metabolism
Ligation adverse effects
Male
Mice
Mice, Inbred C57BL
Peritonitis blood
Peritonitis etiology
Phagocytosis genetics
Phagocytosis physiology
Punctures adverse effects
Sepsis etiology
Transcription Factors genetics
Peritonitis metabolism
Peritonitis prevention & control
Sepsis metabolism
Sepsis prevention & control
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1540-0514
- Volume :
- 52
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Shock (Augusta, Ga.)
- Publication Type :
- Academic Journal
- Accession number :
- 30124596
- Full Text :
- https://doi.org/10.1097/SHK.0000000000001252