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The budding yeast Pex5p receptor directs Fox2p and Cta1p into peroxisomes via its N-terminal region near the FxxxW domain.
- Source :
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Journal of cell science [J Cell Sci] 2018 Sep 07; Vol. 131 (17). Date of Electronic Publication: 2018 Sep 07. - Publication Year :
- 2018
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Abstract
- The import of most of peroxisomal proteins into the lumen of their target organelle is driven by C-terminal (PTS1) or N-terminal (PTS2) signals recognized by the Pex5p or Pex7p receptors, respectively. However, some proteins in budding yeast, such as acyl-CoA oxidase (AOx) and carnitine acetyltransferase (Cat2p), are imported into peroxisomes via an alternative route that does not rely on known PTS signals and involves the Pex5p receptor N-terminal region. Here, we show that two other budding yeast peroxisomal proteins, a multifunctional enzyme from the β-oxidation pathway (Fox2p) and catalase A (Cta1p), both of which contain PTS1, can be imported independently of this signal. The I264K amino acid substitution in Pex5p adjacent to its FxxxW diaromatic motif, previously shown to abolish the import of AOx and Cat2p into peroxisomes, also affects Fox2p and Cta1p import. Moreover, we demonstrate that Pex9p, a newly discovered paralog of Pex5p that was recently implicated in the import of malate synthases in budding yeast, also exhibits weak receptor activity towards Fox2p and Cta1p. These findings indicate the need to re-evaluate the peroxisomal import paradigm.This article has an associated First Person interview with the first author of the paper.<br />Competing Interests: Competing interestsThe authors declare no competing or financial interests.<br /> (© 2018. Published by The Company of Biologists Ltd.)
- Subjects :
- 3-Hydroxyacyl CoA Dehydrogenases genetics
Amino Acid Motifs
Catalase genetics
Enoyl-CoA Hydratase genetics
Peroxisome-Targeting Signal 1 Receptor genetics
Peroxisomes genetics
Protein Domains
Protein Transport
Saccharomyces cerevisiae chemistry
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins chemistry
Saccharomyces cerevisiae Proteins genetics
3-Hydroxyacyl CoA Dehydrogenases metabolism
Catalase metabolism
Enoyl-CoA Hydratase metabolism
Peroxisome-Targeting Signal 1 Receptor chemistry
Peroxisome-Targeting Signal 1 Receptor metabolism
Peroxisomes metabolism
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1477-9137
- Volume :
- 131
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Journal of cell science
- Publication Type :
- Academic Journal
- Accession number :
- 30131444
- Full Text :
- https://doi.org/10.1242/jcs.216986