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Anisotropic organization of circumferential actomyosin characterizes hematopoietic stem cells emergence in the zebrafish.
- Source :
-
ELife [Elife] 2018 Aug 22; Vol. 7. Date of Electronic Publication: 2018 Aug 22. - Publication Year :
- 2018
-
Abstract
- Hematopoiesis leads to the formation of blood and immune cells. Hematopoietic stem cells emerge during development, from vascular components, via a process called the endothelial-to-hematopoietic transition (EHT). Here, we reveal essential biomechanical features of the EHT, using the zebrafish embryo imaged at unprecedented spatio-temporal resolution and an algorithm to unwrap the aorta into 2D-cartography. We show that the transition involves anisotropic contraction along the antero-posterior axis, with heterogenous organization of contractile circumferential actomyosin. The biomechanics of the contraction is oscillatory, with unusually long periods in comparison to other apical constriction mechanisms described so far in morphogenesis, and is supported by the anisotropic reinforcement of junctional contacts. Finally, we show that abrogation of blood flow impairs the actin cytoskeleton, the morphodynamics of EHT cells, and the orientation of the emergence. Overall, our results underline the peculiarities of the EHT biomechanics and the influence of the mechanical forces exerted by blood flow.<br />Competing Interests: ML, SM, SH, FD, JT, JO, PH, AS No competing interests declared<br /> (© 2018, Lancino et al.)
- Subjects :
- Actin Cytoskeleton metabolism
Actins metabolism
Amino Acid Sequence
Animals
Anisotropy
Biomechanical Phenomena
Endothelial Cells cytology
Endothelial Cells metabolism
Hematopoiesis
Hematopoietic Stem Cells cytology
Hemodynamics
Intercellular Junctions metabolism
Models, Biological
Mutation genetics
Myosin Light Chains metabolism
Phenotype
Phosphorylation
Time Factors
Actomyosin metabolism
Hematopoietic Stem Cells metabolism
Zebrafish metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2050-084X
- Volume :
- 7
- Database :
- MEDLINE
- Journal :
- ELife
- Publication Type :
- Academic Journal
- Accession number :
- 30132756
- Full Text :
- https://doi.org/10.7554/eLife.37355