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Antifungal and cytotoxicity activities of Banisteriopsis argyrophylla leaves.

Authors :: Oliveira DM
Silva TFR
Martins MM
de Morais SAL
Chang R
de Aquino FJT
da Silva CV
Teixeira TL
Martins CHG
Moraes TS
Cunha LCS
Pivatto M
de Oliveira A
Source :: The Journal of pharmacy and pharmacology [J Pharm Pharmacol] 2018 Nov; Vol. 70 (11), pp. 1541-1552. Date of Electronic Publication: 2018 Aug 23.
Publication Year :: 2018
Abstract: Objectives: This work aimed to evaluate the antifungal and cytotoxic activity of the EtOH extract and fractions of Banisteriopsis argyrophylla leaves, and to perform the identification of these bioactive metabolites.<br />Methods: The EtOAc fraction (EAF) obtained from the ethanolic extract of B. argyrophylla leaves showed better antifungal potential against Candida spp. In this fraction, ten flavonoids have been identified by UHPLC-ESI-MS <superscript>n</superscript> . Then, EAF was submitted to column chromatography to give four new fractions (A1-A4). The cytotoxicity was determined against Vero cells.<br />Key Findings: The EAF showed better antifungal potential against Candida spp. with minimum inhibitory concentrations (MICs) between 31.25 and 93.75 μg/ml. The (-)-catechin (fraction A1) showed a MIC of 2.83 μg/ml against Candida glabrata. Fractions A2, A3 and A4 were rich in quercetins and kaempferols and showed good inhibitory concentrations (5.86-46.87 μg/ml) against C. albicans, C. glabrata and C. tropicalis.<br />Conclusions: The EtOH extract, fractions and the isolated (-)-catechin showed lower toxicity to Vero cells than cisplatin, used as a positive control. Thus, the leaves of B. argyrophylla are a promising source of antifungal agents.<br /> (© 2018 Royal Pharmaceutical Society.)