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Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1986 Jul; Vol. 83 (14), pp. 5010-4. - Publication Year :
- 1986
-
Abstract
- Among a series of myeloid leukemia cell lines, one (NFS-60) was found to have a rearrangement of the c-myb locus. The rearrangement involved the integration of a retrovirus into the region of the gene corresponding to the sixth exon of the avian c-myb locus. The insertion is associated with the production of a truncated RNA and the introduction of a terminator codon at the juncture of the long terminal repeat and the c-myb locus. The properties of the NSF-60 cells were compared with those of other myeloid cell lines, and the known sequence of differentiation induced by interleukin 3. Similar to other myeloid cell lines, the NFS-60 cells do not terminally differentiate in response to interleukin 3, granulocyte/macrophage, or granulocyte colony-stimulating factor suggesting that the cells are transformed with regard to their ability to differentiate. The NFS-60 cells are totally dependent on interleukin 3 for growth and maintenance of viability in vitro but also proliferate in response to granulocyte colony-stimulating factor. The properties of the cells support the concept that the c-myb protooncogene is involved in the control of normal differentiation of hematopoietic cells.
- Subjects :
- Animals
Cell Differentiation drug effects
Cell Division drug effects
Cell Line
Interleukin-3
Lymphokines pharmacology
Mice
Proto-Oncogene Proteins c-myb
RNA, Messenger analysis
RNA, Neoplasm analysis
DNA, Neoplasm analysis
DNA, Viral analysis
Leukemia Virus, Murine genetics
Leukemia, Experimental genetics
Leukemia, Myeloid genetics
Proto-Oncogene Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 83
- Issue :
- 14
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 3014527
- Full Text :
- https://doi.org/10.1073/pnas.83.14.5010