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Bartonella infections in three species of Microtus: prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success.
- Source :
-
Parasites & vectors [Parasit Vectors] 2018 Aug 30; Vol. 11 (1), pp. 491. Date of Electronic Publication: 2018 Aug 30. - Publication Year :
- 2018
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Abstract
- Background: Bartonella spp. cause persistent bacterial infections in mammals. Although these bacteria are transmitted by blood-feeding arthropods, there is also evidence for vertical transmission in their mammalian hosts. We aimed to determine: (i) the prevalence and diversity of Bartonella spp. in a Microtus spp. community; (ii) whether vertical transmission occurs from infected female voles to their offspring; (iii) the effect of concurrent Babesia microti infection on the success of vertical transmission of Bartonella; and (iv) the impact of congenital infection on pup survival.<br />Results: We sampled 124 Microtus arvalis, 76 Microtus oeconomus and 17 Microtus agrestis. In total, 115 embryos were isolated from 21 pregnant females. In the following year 11 pregnant females were kept until they had given birth and weaned their pups (n = 62). Blood smears and PCR targeting the Bartonella-specific rpoB gene fragment (333bp) were used for the detection of Bartonella. Bartonella DNA was detected in 66.8% (145/217) of the wild-caught voles. Bartonella infection was detected in 81.8% (36/44) of pregnant female voles. Bartonella-positive individuals were identified among the embryos (47.1%; 40/85) and in 54.8% (34/62) of pups. Congenitally acquired Bartonella infections and co-infection with B. microti had no impact on the survival of pups over a 3-week period post partum. Among 113 Bartonella sequences, four species were detected: Bartonella taylorii, Bartonella grahamii, Bartonella doshiae and a Bartonella rochalimae-like genotype. Bartonella taylorii clade B was the dominant species in wild-caught voles (49%), pregnant females (47%), their embryos (85%), dams (75%) and pups (95%).<br />Conclusions: High prevalence of Bartonella spp. infection maintained in Microtus spp. community is followed by a high rate of vertical transmission of several rodent species of Bartonella in three species of naturally infected voles, M. arvalis, M. oeconomus and M. agrestis. Congenitally acquired Bartonella infection does not affect the survival of pups. Co-infection with B. microti does not affect the effectiveness of the vertical transmission of Bartonella in voles. Bartonella taylorii clade B was found to be the dominant species in wild-caught voles, including pregnant females and dams, and in their offspring, and was also found to be the most successful in vertical transmission.
- Subjects :
- Animals
Babesia microti isolation & purification
Babesia microti physiology
Babesiosis epidemiology
Bartonella physiology
Bartonella Infections congenital
Bartonella Infections epidemiology
Bartonella Infections microbiology
Coinfection microbiology
Coinfection parasitology
Embryo, Mammalian microbiology
Embryo, Mammalian parasitology
Female
Genotype
Prevalence
Rodent Diseases epidemiology
Rodent Diseases microbiology
Rodent Diseases parasitology
Arvicolinae microbiology
Arvicolinae parasitology
Babesiosis parasitology
Bartonella genetics
Bartonella Infections transmission
Genetic Variation
Infectious Disease Transmission, Vertical
Subjects
Details
- Language :
- English
- ISSN :
- 1756-3305
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Parasites & vectors
- Publication Type :
- Academic Journal
- Accession number :
- 30165879
- Full Text :
- https://doi.org/10.1186/s13071-018-3047-6