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Selenocysteine-Specific Mass Spectrometry Reveals Tissue-Distinct Selenoproteomes and Candidate Selenoproteins.
- Source :
-
Cell chemical biology [Cell Chem Biol] 2018 Nov 15; Vol. 25 (11), pp. 1380-1388.e4. Date of Electronic Publication: 2018 Aug 30. - Publication Year :
- 2018
-
Abstract
- Selenoproteins, defined by the presence of selenocysteines (Sec), play important roles in a wide range of biological processes. All known selenoproteins are marked by the presence of Sec insertion sequence (SECIS) at their mRNA. The lack of an effective analytical method has hindered our ability to explore the selenoproteome and new selenoproteins beyond SECIS. Here, we develop a Sec-specific mass spectrometry-based technique, termed "SecMS," which allows the systematic profiling of selenoproteomes by selective alkylation of Sec. Using SecMS, we quantitatively characterized the age- and stress-regulated selenoproteomes for nine tissues from mice of different ages and mammalian cells, demonstrating tissue-specific selenoproteomes and an age-dependent decline in specific selenoproteins in brains and hearts. We established an integrated platform using SecMS and SECIS-independent selenoprotein (SIS) database and further identified five candidate selenoproteins. The application of this integrated platform provides an effective strategy to explore the selenoproteome independent of SECIS.<br /> (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Subjects :
- Aging
Alkylation
Amino Acid Sequence
Animals
Brain metabolism
Brain Chemistry
Cell Line
Female
HEK293 Cells
Humans
Male
Mice, Inbred C57BL
Myocardium chemistry
Myocardium metabolism
Proteome chemistry
Proteome metabolism
Proteomics methods
Selenocysteine metabolism
Selenoproteins metabolism
Stress, Physiological
Mass Spectrometry methods
Selenocysteine analysis
Selenoproteins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2451-9448
- Volume :
- 25
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Cell chemical biology
- Publication Type :
- Academic Journal
- Accession number :
- 30174312
- Full Text :
- https://doi.org/10.1016/j.chembiol.2018.08.006