Gel electrophoresis of human sperm: a simple method for evaluating sperm protein quality.

Background: The limitations of conventional sperm analyses have highlighted the need for additional means of evaluating sperm quality.
Methods: In a study of a cohort of 245 men with known conventional sperm parameters, one-dimensional PAGE was used to monitor protein content and quality in samples from individual ejaculates.
Results: The sperm protein content varied markedly from sample to another, especially in the high-molecular-weight range. The intensity of the 80-110 kDa bands was correlated with progressive motility ( r  = 0.15, p  = 0.015 ) and was significantly higher ( p  = 0.0367) in the group of men with conventional parameters above the World Health Organization's 2010 reference values than in the group with at least one subnormal parameter (i.e. semen volume, sperm concentration, sperm count per ejaculate, progressive motility, proportion of normal forms or multiple anomaly index below the lower reference value). Using mass spectrometry, the 80-110 kDa bands were found to correspond primarily to three proteins from the flagellum's fibrous sheath: A-kinase anchor protein 4, A-kinase anchor protein 3, and spermatogenic cell-specific type 1 hexokinase.
Conclusion: One-dimensional PAGE constitutes a simple, rapid, reliable, inexpensive method for analyzing proteins associated with sperm motility in individual human ejaculates.
Competing Interests: Human ejaculates were obtained (following the provision of written, informed consent) from the Assisted Reproduction Unit at Lille University Medical Center (Lille, France). All the donors (age range: 30–37) had consented to use of the ejaculates for research purposes. Furthermore, the study protocol was approved by the local investigational review board (Comité de Protection des Personnes Nord Ouest IV, Lille, France; reference HP 16/53). The study was conducted in accordance with Lille University Medical Center’s regulations on clinical research.Not applicable.BL, MV and SN are co-founders of SPQI, a spin-off company involved in biomarker research in male infertility. JF, SJ, SN and MV are cited as inventors on a patent application (Patent EP13306212.5).Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.