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Aplysin Protects Against Alcohol-Induced Liver Injury Via Alleviating Oxidative Damage and Modulating Endogenous Apoptosis-Related Genes Expression in Rats.
- Source :
-
Journal of food science [J Food Sci] 2018 Oct; Vol. 83 (10), pp. 2612-2621. Date of Electronic Publication: 2018 Sep 07. - Publication Year :
- 2018
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Abstract
- We investigated the protective effects and possible mechanisms of Aplysin against alcohol-induced liver injury. Rats were given daily either alcohol only (alcohol model group; 8 to 12 mL/kg body weight), one of three doses of Aplysin (50, 100, or 150 mg/kg Aplysin) plus alcohol, or volume-matched saline. After 6 weeks, the effects of Aplysin were assessed in terms of changes in histology, biochemical indices, and DNA oxidative damage. Potential mechanisms were analyzed through measurements of lipid peroxidation, antioxidant defense systems, expression of cytochrome P450 2E1, and expression of apoptosis-related genes. We found that Aplysin significantly protected the liver against alcohol-induced oxidative injury, evidenced by improved hepatic histological structure, inhibited alcohol-induced elevation of serum biochemical indices, attenuated extents of hepatocellular DNA damage. At a mechanistic level, Aplysin alleviated alcohol-induced oxidative stress as illustrated by the revivification of erythrocyte membrane fluidity, the attenuation of glutathione depletion, the restoration of antioxidase activities, and reduced malondialdehyde overproduction. Furthermore, the mRNA levels of Bax, cytochrome c, and cytochrome P450 2E1 were significantly down-regulated, whereas those of Bcl-2 and caspase-9 and caspase-3 were markedly up-regulated. These findings suggest that Aplysin provides significant protection against alcohol-induced liver injury, possibly through alleviating oxidative damage and modulating endogenous apoptosis-related genes expression.<br />Practical Application: Many natural components derived from alga have been used in the food, cosmetics, and biomedicine industries. Aplysin, a marine bromosesquiterpene, was extracted from the red alga Laurencia tristicha, which could effectively protect against alcohol-induced liver injury, might be a potential natural sources for preventing alcoholic liver damage.<br /> (© 2018 Institute of Food Technologists®.)
- Subjects :
- Animals
Antioxidants pharmacology
Caspase 3 metabolism
Cytochrome P-450 CYP2E1 metabolism
DNA Damage drug effects
Erythrocyte Membrane drug effects
Gene Expression drug effects
Glutathione metabolism
Laurencia
Lipid Peroxidation drug effects
Liver drug effects
Male
Malondialdehyde metabolism
Plant Extracts
Proto-Oncogene Proteins c-bcl-2 metabolism
Rats
Rats, Wistar
Apoptosis drug effects
Chemical and Drug Induced Liver Injury drug therapy
Ethanol adverse effects
Hydrocarbons, Brominated pharmacology
Oxidative Stress drug effects
Sesquiterpenes pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1750-3841
- Volume :
- 83
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of food science
- Publication Type :
- Academic Journal
- Accession number :
- 30192013
- Full Text :
- https://doi.org/10.1111/1750-3841.14320