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In Vitro Selection of Single-Domain Antibody (VHH) Using cDNA Display.

Authors :
Nemoto N
Kumachi S
Arai H
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1827, pp. 269-285.
Publication Year :
2018

Abstract

Single-domain antibody (e.g., Nanobody, VHH antibody) is a promising scaffold for therapeutic and diagnostic reagents. To expand the range of target molecules, in vitro selection using cell-free display technologies such as cDNA display is useful and powerful because of their huge libraries and robust stability. We provide technical details for in vitro selection of single-domain antibodies using cDNA display.

Subjects

Subjects :
Animals
Cross-Linking Reagents chemistry
DNA, Complementary genetics
Light
Puromycin chemistry
RNA, Messenger genetics
RNA, Messenger metabolism
Rabbits
Transcription, Genetic
Cell Surface Display Techniques methods
DNA, Complementary metabolism
Single-Domain Antibodies metabolism

Details

Language :
English
ISSN :
1940-6029
Volume :
1827
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
30196502
Full Text :
https://doi.org/10.1007/978-1-4939-8648-4_14
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