[Construction of A Genetic Linkage Map in Salvia miltiorrhiza].

Objective: On the basis of previous studies, to construct a genetic map of Salvia miltiorrhiza by using EST-SSR primers, to build a platform for positioning important traits relating to genes, the cloning and molecular marker-assisted in breeding of new varieties of Salvia miltiorrhiza.
Methods: A total of 411 EST-SSR primers were used for PCR amplification to screen polymorphic markers in F1 mapping population derived from a cross between Salvia miltiorrhiza, two parents of which were the cultivars of ZH74 and BH18. Combined with the molecular marker data of previous studies, Joinmap 4. 0 software was used for map integration.
Results: 411EST-SSR primers were screened from two parents, a total of 328 pairs of primers were amplified stable products,164 pairs of polymorphic primers were obtained.
Conclusion: A linkage map of Salvia miltiorrhiza with 150 marker high density genetic is constructed.