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Removal of Microcystin-LR by a Novel Native Effective Bacterial Community Designated as YFMCD4 Isolated from Lake Taihu.
- Source :
-
Toxins [Toxins (Basel)] 2018 Sep 08; Vol. 10 (9). Date of Electronic Publication: 2018 Sep 08. - Publication Year :
- 2018
-
Abstract
- Microcystin-LR (MC-LR) is the most toxic and frequently detected monocyclic heptapeptide hepatotoxin produced by cyanobacteria, which poses a great threat to the natural ecosystem and public health. It is very important to seek environment-friendly and cost-efficient methods to remove MC-LR in water. In this study, the MC-degrading capacities of a novel indigenous bacterial community designated as YFMCD4 and the influence of environmental factors including various temperatures, MC concentrations and pH on the MC-degrading activities were investigated utilizing high-performance liquid chromatography (HPLC). In addition, the MC-degrading mechanism of YFMCD4 was also studied using HPLC coupled with a mass spectrometry equipped with electrospray ionization interface (HPLC-ESI-MS). The data showed MC-LR was completely removed at the maximum rate of 0.5 µg/(mL·h) under the optimal condition by YFMCD4. Two pure bacterial strains Alcaligenes faecalis and Stenotrophomonas acidaminiohila were isolated from YFMCD4 degraded MC-LR at a slower rate. The MC-degrading rates of YFMCD4 were significantly affected by different temperatures, pH and MC-LR concentrations. Two intermediates of a tetrapeptide and Adda appeared in the degradation process. These results illustrate that the novel YFMCD4 is one of the highest effective MC-degrading bacterial community, which can completely remove MC-LR and possesses a significant potential to treat water bodies contaminated by MC-LR.
- Subjects :
- China
Chromatography, High Pressure Liquid
Hydrogen-Ion Concentration
Lakes
Marine Toxins
Spectrometry, Mass, Electrospray Ionization
Temperature
Water Microbiology
Water Purification methods
Alcaligenes faecalis metabolism
Microcystins metabolism
Water Pollutants metabolism
Xanthomonadaceae metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2072-6651
- Volume :
- 10
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Toxins
- Publication Type :
- Academic Journal
- Accession number :
- 30205544
- Full Text :
- https://doi.org/10.3390/toxins10090363