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Identification of Changing Ribosome Protein Compositions using Mass Spectrometry.
- Source :
-
Proteomics [Proteomics] 2018 Oct; Vol. 18 (20), pp. e1800217. - Publication Year :
- 2018
-
Abstract
- The regulatory role of the ribosome in gene expression has come into sharper focus. It has been proposed that ribosomes are dynamic complexes capable of changing their protein composition in response to environmental stimuli. MS is applied to identify quantitative changes in the protein composition of S. cerevisiae 80S ribosomes in response to different environmental stimuli. Using quantitative MS, it is found that the paralog yeast ribosomal proteins RPL8A (eL8A) and RPL8B (eL8B) change their relative proportions in the 80S ribosome when yeast is switched from growth in glucose to glycerol. By using yeast genetics and polysome profiling, it is shown that yeast ribosomes containing either RPL8A or RPL8B are not functionally interchangeable. The quantitative proteomic data support the hypothesis that ribosomes are dynamic complexes that alter their composition and functional activity in response to changes in growth or environmental conditions.<br /> (© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Cryoprotective Agents pharmacology
Glucose pharmacology
Glycerol pharmacology
Mass Spectrometry
Ribosomal Proteins chemistry
Ribosomes chemistry
Saccharomyces cerevisiae drug effects
Saccharomyces cerevisiae growth & development
Sweetening Agents pharmacology
Polyribosomes metabolism
Ribosomal Proteins metabolism
Ribosomes metabolism
Saccharomyces cerevisiae metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1615-9861
- Volume :
- 18
- Issue :
- 20
- Database :
- MEDLINE
- Journal :
- Proteomics
- Publication Type :
- Academic Journal
- Accession number :
- 30211483
- Full Text :
- https://doi.org/10.1002/pmic.201800217