Back to Search
Start Over
Transcript Profiling of MRSA Biofilms Treated with a Halogenated Phenazine Eradicating Agent: A Platform for Defining Cellular Targets and Pathways Critical to Biofilm Survival.
- Source :
-
Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2018 Nov 19; Vol. 57 (47), pp. 15523-15528. Date of Electronic Publication: 2018 Oct 25. - Publication Year :
- 2018
-
Abstract
- Bacterial biofilms are surface-attached communities of non-replicating bacteria innately tolerant to antibiotics. Biofilms display differential gene expression profiles and physiologies as compared to their planktonic counterparts; however, their biology remains largely unknown. In this study, we used a halogenated phenazine (HP) biofilm eradicator in transcript profiling experiments (RNA-seq) to define cellular targets and pathways critical to biofilm viability. WoPPER analysis with time-course validation (RT-qPCR) revealed that HP-14 induces rapid iron starvation in MRSA biofilms, as evident by the activation of iron-acquisition gene clusters in 1 hour. Serine proteases and oligopeptide transporters were also found to be up-regulated, whereas glycolysis, arginine deiminase, and urease gene clusters were down-regulated. KEGG analysis revealed that HP-14 impacts metabolic and ABC transporter functional pathways. These findings suggest that MRSA biofilm viability relies on iron homeostasis.<br /> (© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Anti-Bacterial Agents chemistry
Anti-Bacterial Agents pharmacology
Halogenation
Humans
Iron metabolism
Methicillin-Resistant Staphylococcus aureus drug effects
Phenazines chemistry
Phenazines pharmacology
Signal Transduction drug effects
Staphylococcal Infections drug therapy
Staphylococcal Infections microbiology
Biofilms drug effects
Methicillin-Resistant Staphylococcus aureus genetics
Methicillin-Resistant Staphylococcus aureus physiology
Transcriptome drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1521-3773
- Volume :
- 57
- Issue :
- 47
- Database :
- MEDLINE
- Journal :
- Angewandte Chemie (International ed. in English)
- Publication Type :
- Academic Journal
- Accession number :
- 30230671
- Full Text :
- https://doi.org/10.1002/anie.201809785