Upstream Hedgehog signaling components are exported in exosomes of cervical cancer cell lines.

Authors :: Bhat A
Sharma A
Bharti AC
Source :: Nanomedicine (London, England) [Nanomedicine (Lond)] 2018 Sep; Vol. 13 (17), pp. 2127-2138. Date of Electronic Publication: 2018 Sep 28.
Publication Year :: 2018
Abstract: Aim: To investigate export of Hedgehog pathway (Hh) proteins Patched1, Smoothened, Sonic hedgehog and Indian hedgehog in cervical cancer cell line (CaCx) exosomes.<br />Methods: Exosomes were isolated and characterized by Western blotting, scanning electron microscopy and in a colorimetric assay. Nucleic acids (RNA, DNA) and protein content of exosomes were analyzed. Hh pathway proteins in exosomes were detected using Western blotting.<br />Results: CaCx secrete bio-macromolecule (DNA, RNA and proteins) enriched exosomes. CaCx exosomes contained higher amount of RNA with respect to DNA. CaCx preferentially exported Hh proteins (Patched1, Smoothened,  Sonic hedgehog, Indian hedgehog) in their exosomes. Cellular uptake assay revealed rapid internalization of CaCx exosomes in human umbilical vein endothelial cells.<br />Conclusion: Our study showed that Hh proteins are exported in CaCx exosomes.

Subjects :: Cell Line, Tumor
Female
Humans
Patched-1 Receptor metabolism
Receptors, Cell Surface metabolism
Signal Transduction
Smoothened Receptor metabolism
Exosomes metabolism
Hedgehog Proteins metabolism
Uterine Cervical Neoplasms metabolism

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