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Immunomagnetic antibody plus aptamer pseudo-DNA nanocatenane followed by rolling circle amplication for highly-sensitive CTC detection.
- Source :
-
Biosensors & bioelectronics [Biosens Bioelectron] 2018 Dec 30; Vol. 122, pp. 239-246. Date of Electronic Publication: 2018 Sep 07. - Publication Year :
- 2018
-
Abstract
- Biosensing and detecting the rare circulating tumor cells (CTCs) in complex blood samples are a great challenge but necessary for cancer metastasis prevention. Here we show a novel highly-sensitive biosensing system for detecting CTCs in whole blood. The system is composed of Her2-coated immunomagnetic beads and an anti-EpCAM aptamer assembled pseudo-DNA nanocatenane (PDN) for dual targeting and separating CTCs, in conjunction with the rolling circle amplification (RCA) and molecular beacon (MB) system for CTCs signal amplification. The Her-2-coated beads separated CTCs from blood after their elution from a magnetic column. The unique PDN, which is a tailor-designed self-assembly of three circular DNAs that are inter-locked with independent and non-interfered templates for periodic RCA process, binds EpCAM-rich CTCs. In the presence of the RCA primer, phi29 DNA polymerase and MB, the system collaboratively generated the amplified fluorescent signals for highly-sensitive detection of CTCs. Through this system, we achieved the limit of detection less than 10 CTCs/mL blood, and quantified the number of CTCs in patient blood, which is proportional to the patient cancer status. Our technique is highly-sensitive, practicable and convenient enough for clinical detection of breast CTCs.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Subjects :
- Antibodies, Immobilized chemistry
Breast Neoplasms diagnosis
Cell Line, Tumor
Epithelial Cell Adhesion Molecule analysis
Female
Humans
Neoplastic Cells, Circulating chemistry
Nucleic Acid Amplification Techniques methods
Receptor, ErbB-2 analysis
Aptamers, Nucleotide chemistry
Biosensing Techniques methods
Breast Neoplasms blood
Immunomagnetic Separation methods
Neoplastic Cells, Circulating pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4235
- Volume :
- 122
- Database :
- MEDLINE
- Journal :
- Biosensors & bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 30267982
- Full Text :
- https://doi.org/10.1016/j.bios.2018.09.025