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Cyclic stretch increases mitochondrial biogenesis in a cardiac cell line.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2018 Nov 02; Vol. 505 (3), pp. 768-774. Date of Electronic Publication: 2018 Oct 05. - Publication Year :
- 2018
-
Abstract
- Unlike stable and immobile cell line conditions, animal hearts contract and relax to pump blood throughout the body. Mitochondria play an essential role by producing biological energy molecules to maintain heart function. In this study, we assessed the effect of heart mimetic cyclic stretch on mitochondria in a cardiac cell line. To mimic the geometric and biomechanical conditions surrounding cells in vivo, cyclic stretching was performed on HL-1 murine cardiomyocytes seeded onto an elastic micropatterned substrate (10% elongation, 0.5 Hz, 4 h/day). Cell viability, semi-quantitative Q-PCR, and western blot analyses were performed in non-stimulated control and cyclic stretch stimulated HL-1 cell lines. Cyclic stretch significantly increased the expression of mitochondria biogenesis-related genes (TUFM, TFAM, ERRα, and PGC1-α) and mitochondria oxidative phosphorylation-related genes (PHB1 and CYTB). Western blot analysis confirmed that cyclic stretch increased protein levels of mitochondria biogenesis-related proteins (TFAM, and ERRα) and oxidative phosphorylation-related proteins (NDUFS1, UQCRC, and PHB1). Consequently, cyclic stretch increased mitochondrial mass and ATP production in treated cells. Our results suggest that cyclic stretch transcriptionally enhanced mitochondria biogenesis and oxidative phosphorylation without detrimental effects in a cultured cardiac cell line.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Subjects :
- Adenosine Triphosphate metabolism
Animals
Cell Line
Cell Survival
Gene Expression
Mice
Mitochondria, Heart genetics
Mitochondrial Proteins genetics
Mitochondrial Proteins metabolism
Myocytes, Cardiac cytology
Oxidative Phosphorylation
Mitochondria, Heart metabolism
Myocytes, Cardiac metabolism
Organelle Biogenesis
Stress, Mechanical
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 505
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 30297109
- Full Text :
- https://doi.org/10.1016/j.bbrc.2018.10.003