Back to Search
Start Over
Determination of confocal profile and curved focal plane for OCT mapping of the attenuation coefficient.
- Source :
-
Biomedical optics express [Biomed Opt Express] 2018 Oct 01; Vol. 9 (10), pp. 5084-5099. Date of Electronic Publication: 2018 Oct 01 (Print Publication: 2018). - Publication Year :
- 2018
-
Abstract
- The attenuation coefficient has proven to be a useful tool in numerous biological applications, but accurate calculation is dependent on the characterization of the confocal effect. This study presents a method to precisely determine the confocal effect and its focal plane within a sample by examining the ratio of two optical coherence tomography (OCT) images. The method can be employed to produce a single-value estimate, or a 2D map of the focal plane accounting for the curvature or tilt within the sample. Furthermore, this method is applicable to data obtained with both high numerical aperture (NA) and low-NA lenses, thereby furthering the applicability of the attenuation coefficient to high-NA OCT data. We test and validate this method using standard samples of Intralipid 20% and 5%, improving the accuracy to 99% from 65% compared to the traditional method and preliminarily show applicability to real biological data of glioblastoma acquired in vivo in a murine model.<br />Competing Interests: The authors declare that there are no conflicts of interest related to this article.
Details
- Language :
- English
- ISSN :
- 2156-7085
- Volume :
- 9
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Biomedical optics express
- Publication Type :
- Academic Journal
- Accession number :
- 30319923
- Full Text :
- https://doi.org/10.1364/BOE.9.005084