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Identification and characterization of two zebrafish Twik related potassium channels, Kcnk2a and Kcnk2b.
- Source :
-
Scientific reports [Sci Rep] 2018 Oct 17; Vol. 8 (1), pp. 15311. Date of Electronic Publication: 2018 Oct 17. - Publication Year :
- 2018
-
Abstract
- KCNK2 is a 2 pore domain potassium channel involved in maintaining cellular membrane resting potentials. Although KCNK2 is regarded as a mechanosensitive ion channel, it can also be gated chemically. Previous research indicates that KCNK2 expression is particularly enriched in neuronal and cardiac tissues. In this respect, KCNK2 plays an important role in neuroprotection and has also been linked to cardiac arrhythmias. KCNK2 has subsequently become an attractive pharmacologic target for developing preventative/curative strategies for neuro/cardio pathophysiological conditions. Zebrafish represent an important in vivo model for rapidly analysing pharmacological compounds. We therefore sought to identify and characterise zebrafish kcnk2 to allow this model system to be incorporated into therapeutic research. Our data indicates that zebrafish possess two kcnk2 orthologs, kcnk2a and kcnk2b. Electrophysiological analysis of both zebrafish Kcnk2 orthologs shows that, like their human counterparts, they are activated by different physiological stimuli such as mechanical stretch, polyunsaturated fatty acids and intracellular acidification. Furthermore, both zebrafish Kcnk2 channels are inhibited by the human KCNK2 inhibitory peptide spadin. Taken together, our results demonstrate that both Kcnk2a and Kcnk2b share similar biophysiological and pharmacological properties to human KCNK2 and indicate that the zebrafish will be a useful model for developing KCNK2 targeting strategies.
- Subjects :
- Animals
Antidepressive Agents, Second-Generation pharmacology
Cells, Cultured
Fluoxetine pharmacology
HEK293 Cells
Humans
Ion Channel Gating drug effects
Ion Channel Gating genetics
Membrane Potentials drug effects
Membrane Potentials genetics
Membrane Potentials physiology
Neuroprotective Agents pharmacology
Peptides pharmacology
Potassium Channels, Tandem Pore Domain genetics
Potassium Channels, Tandem Pore Domain physiology
Protein Isoforms genetics
Protein Isoforms physiology
Riluzole pharmacology
Zebrafish genetics
Zebrafish Proteins genetics
Zebrafish Proteins physiology
Ion Channel Gating physiology
Potassium Channels, Tandem Pore Domain metabolism
Protein Isoforms metabolism
Zebrafish metabolism
Zebrafish Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 30333618
- Full Text :
- https://doi.org/10.1038/s41598-018-33664-9