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Detection of Endogenous Phosphatidylinositol 4,5-bisphosphate in Phytophthora cinnamomi.

Authors :
Howard JM
Beech PL
Callahan DL
Source :
Lipids [Lipids] 2018 Aug; Vol. 53 (8), pp. 835-839. Date of Electronic Publication: 2018 Oct 17.
Publication Year :
2018

Abstract

Plant diseases caused by Phytophthora species are serious threats to agriculture and the natural environment. Genome sequencing has revealed the lack of a gene for canonical phospholipase C (PLC), an enzyme that was hitherto thought to be ubiquitous in eukaryotes. PLC acts in the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdIns-4,5-P <subscript>2</subscript> ), a membrane-bound phospholipid critical for signal initiation in many cellular processes. Previous studies have not provided evidence of endogenous PtdIns-4,5-P <subscript>2</subscript> in Phytophthora and, in the absence of canonical PLC, argued for redundancy or loss in the PLC pathway in Phytophthora. Using liquid chromatography mass spectrometry, we have detected endogenous PtdIns-4,5-P <subscript>2</subscript> in Phytophthora cinnamomi. This is the first identification of the phospholipid in the genus, and is significant because it indicates that the signal transduction pathway of the PLC product, inositol 1,4,5-trisphosphate (IP <subscript>3</subscript> ), may have been retained in Phytophthora incorporating an as-yet unidentified homolog or analog of PLC.<br /> (© 2018 AOCS.)

Details

Language :
English
ISSN :
1558-9307
Volume :
53
Issue :
8
Database :
MEDLINE
Journal :
Lipids
Publication Type :
Academic Journal
Accession number :
30334270
Full Text :
https://doi.org/10.1002/lipd.12093