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CD32 Expression is not Associated to HIV-DNA content in CD4 cell subsets of individuals with Different Levels of HIV Control.
- Source :
-
Scientific reports [Sci Rep] 2018 Oct 19; Vol. 8 (1), pp. 15541. Date of Electronic Publication: 2018 Oct 19. - Publication Year :
- 2018
-
Abstract
- A recent study has pointed out to CD32a as a potential biomarker of HIV-persistent CD4 cells. We have characterized the level and phenotype of CD32+ cells contained in different subsets of CD4 T-cells and its potential correlation with level of total HIV-DNA in thirty HIV patients (10 typical progressors naïve for cART, 10 cART-suppressed patients, and 10 elite controllers). Total HIV-DNA was quantified in different subsets of CD4 T-cells: Trm and pTfh cells. Level and immunephenotype of CD32+ cells were analyzed in these same subsets by flow cytometry. CD32 expression in Trm and pTfh subsets was similar in the different groups, and there was no significant correlation between the level of total HIV-DNA and the level of CD32 expression in these subsets. However, total HIV-DNA level was correlated with expression of CD127 (rho = -0.46, p = 0.043) and of CCR6 (rho = -0.418, p = 0.027) on CD32+ cells. Our results do not support CD32 as a biomarker of total HIV-DNA content. However, analyzing the expression of certain markers by CD32+ cells could improve the utility of this marker in the clinical setting, prompting the necessity of further studies to both validate our results and to explore the potential utility of certain markers expressed by CD32+ cells.
- Subjects :
- Adult
CD4-Positive T-Lymphocytes chemistry
Female
Flow Cytometry
Gene Expression Profiling
HIV Infections drug therapy
HIV Infections virology
Humans
Male
Middle Aged
T-Lymphocyte Subsets chemistry
T-Lymphocyte Subsets virology
Viral Load
Anti-Retroviral Agents therapeutic use
CD4-Positive T-Lymphocytes virology
DNA, Viral analysis
Gene Expression
HIV growth & development
HIV Infections pathology
Receptors, IgG analysis
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 30341387
- Full Text :
- https://doi.org/10.1038/s41598-018-33749-5