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Engineered cyclodextrin glucanotransferases from Bacillus sp. G-825-6 produce large-ring cyclodextrins with high specificity.

Engineered cyclodextrin glucanotransferases from Bacillus sp. G-825-6 produce large-ring cyclodextrins with high specificity.

Authors :
Sonnendecker C
Melzer S
Zimmermann W
Source :
MicrobiologyOpen [Microbiologyopen] 2019 Jun; Vol. 8 (6), pp. e00757. Date of Electronic Publication: 2018 Oct 25.
Publication Year :
2019

Abstract

Cyclodextrin glucanotransferases (CGTases) synthesize cyclic oligosaccharides (cyclodextrins, CD) from starch. A CGTase from Bacillus sp. G-825-6 was engineered by site-directed mutagenesis at two positions by the construction of the variants Y183W, Y183R, D358R, Y183W/D358R, and Y183R/D358R. Among CD composed of 7-12 glucose units (CD7-CD12), Y183W mainly produced CD8. Y183R had completely lost its ability to synthesize CD7, and CD8 and the larger CD were the only cyclic oligosaccharides produced. D358R also formed mainly CD8-CD12 during a reaction time of 24 hr. The double mutant Y183W/D358R showed combined characteristics of the single mutations with very low CD7 cyclization activity and an increased formation of the larger CD. The results show that CGTases synthesizing mainly CD8-CD12 can be constructed allowing a convenient production of larger CD in significant amounts as host molecules in supramolecular complexing reactions.<br /> (© 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.)

Details

Language :
English
ISSN :
2045-8827
Volume :
8
Issue :
6
Database :
MEDLINE
Journal :
MicrobiologyOpen
Publication Type :
Academic Journal
Accession number :
30358941
Full Text :
https://doi.org/10.1002/mbo3.757