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Engineering bacteria for biogenic synthesis of chalcogenide nanomaterials.
- Source :
-
Microbial biotechnology [Microb Biotechnol] 2019 Jan; Vol. 12 (1), pp. 161-172. Date of Electronic Publication: 2018 Oct 17. - Publication Year :
- 2019
-
Abstract
- Microbes naturally build nanoscale structures, including structures assembled from inorganic materials. Here, we combine the natural capabilities of microbes with engineered genetic control circuits to demonstrate the ability to control biological synthesis of chalcogenide nanomaterials in a heterologous host. We transferred reductase genes from both Shewanella sp. ANA-3 and Salmonella enterica serovar Typhimurium into a heterologous host (Escherichia coli) and examined the mechanisms that regulate the properties of biogenic nanomaterials. Expression of arsenate reductase genes and thiosulfate reductase genes in E. coli resulted in the synthesis of arsenic sulfide nanomaterials. In addition to processing the starting materials via redox enzymes, cellular components also nucleated the formation of arsenic sulfide nanomaterials. The shape of the nanomaterial was influenced by the bacterial culture, with the synthetic E. coli strain producing nanospheres and conditioned media or cultures of wild-type Shewanella sp. producing nanofibres. The diameter of these nanofibres also depended on the biological context of synthesis. These results demonstrate the potential for biogenic synthesis of nanomaterials with controlled properties by combining the natural capabilities of wild microbes with the tools from synthetic biology.<br /> (© 2018 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.)
- Subjects :
- Cloning, Molecular
Gene Expression
Oxidoreductases genetics
Oxidoreductases metabolism
Recombinant Proteins genetics
Recombinant Proteins metabolism
Salmonella typhimurium enzymology
Salmonella typhimurium genetics
Shewanella enzymology
Shewanella genetics
Arsenicals metabolism
Escherichia coli genetics
Escherichia coli metabolism
Metabolic Engineering methods
Nanostructures
Sulfides metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1751-7915
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Microbial biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 30369058
- Full Text :
- https://doi.org/10.1111/1751-7915.13320