Back to Search
Start Over
Estrogen ameliorates allergic airway inflammation by regulating activation of NLRP3 in mice.
- Source :
-
Bioscience reports [Biosci Rep] 2019 Jan 08; Vol. 39 (1). Date of Electronic Publication: 2019 Jan 08 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- Background: Estrogen has been suggested to play a protective role against airway inflammations, such as asthma. In these processes, the inflammasome nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain containing 3 (NLRP3) partly accounts for the activation of pro-inflammatory factors. The aim of the present study was to investigate whether NLRP3 was involved in the protective effect of estrogen against allergic airway inflammation. Methods: An ovariectomy was performed on female C57BL/6 mice; some were sham-operated (sham). We then sensitized and challenged them with ovalbumin (OVA) to establish an airway inflammation model. Meanwhile, some mice were treated with 17β-estradiol (E2) for 28 days. Results: The expression of NLRP3 inflammasome and its downstream products, caspase-1 and the pro-inflammatory cytokine interleukin (IL)-1β (IL-1β), increased concomitantly with OVA-challenged airway inflammation and decreased with the expression of estrogen receptor β (ERβ). In addition, treating ovariectomized (OVX) mice with E2 dramatically ameliorated airway inflammation via such mechanisms as leukocyte recruitment, mucus production, and secretion of pro-inflammatory cytokines other than IL-18 in bronchoalveolar lavage (BAL) fluid (BALF). Furthermore, E2 suppressed both the mRNA expression and protein expression of NLRP3, caspase-1, and IL-1β. In summary, our study showed that NLRP3 inflammasome activation and pro-inflammatory cytokine production markedly increased in OVA-induced airway inflammation, and E2 effectively abrogated such inflammation by regulating the activation of NLRP3.<br /> (© 2018 The Author(s).)
- Subjects :
- Animals
Asthma chemically induced
Bronchoalveolar Lavage Fluid cytology
Caspase 1 metabolism
Disease Models, Animal
Estradiol metabolism
Estrogen Receptor beta metabolism
Female
Inflammasomes drug effects
Inflammasomes metabolism
Inflammation chemically induced
Interleukin-1beta metabolism
Mice
Mice, Inbred C57BL
Ovalbumin pharmacology
Ovariectomy methods
Respiratory System drug effects
Asthma metabolism
Estrogens metabolism
Inflammation metabolism
NLR Family, Pyrin Domain-Containing 3 Protein metabolism
Respiratory System metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1573-4935
- Volume :
- 39
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Bioscience reports
- Publication Type :
- Academic Journal
- Accession number :
- 30373775
- Full Text :
- https://doi.org/10.1042/BSR20181117