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LncRNA NEAT1 promotes colorectal cancer cell proliferation and migration via regulating glial cell-derived neurotrophic factor by sponging miR-196a-5p.
- Source :
-
Acta biochimica et biophysica Sinica [Acta Biochim Biophys Sin (Shanghai)] 2018 Dec 01; Vol. 50 (12), pp. 1190-1199. - Publication Year :
- 2018
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Abstract
- Colorectal cancer (CRC) is the one of the most common malignant tumors worldwide. Recent years, widespread long non-coding RNAs (lncRNAs) have been discovered and are known to regulate gene expression in cancers. However, the roles and underlying mechanisms of lncRNA in CRC remain largely unclear. Here, we firstly revealed that repression of lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) inhibited cell proliferation and migration in HCT116 cells and overexpression of NEAT1 promoted cell proliferation and migration in SW480 cells using CCK8 assay and transwell assay. Then, we found that suppression of NEAT1 increased the miR-196a-5p expression in HCT116 cells, while elevation of NEAT1 decreased the miR-196a-5p expression in SW480 cells using qPCR assay. Furthermore, miR-196a-5p could bind to the predicted binding site of NEAT1. We then found that miR-196a-5p was involved in the role of NEAT1 in CRCs. In addition, we demonstrated that miR-196a-5p mimics inhibited the glial cell-derived neurotrophic factor (GDNF) expression in HCT116 cells and meanwhile, miR-196a-5p inhibitor promoted GDNF expression in SW480 cells using qPCR and western blot analysis. Then, we proved that miR-196a-5p exerted its function via regulating GDNF expression in CRCs. Ultimately, our study demonstrated that NEAT1 exerted its role via miR-196a-5p/GDNF axis in CRCs. In summary, this work provided the first evidence of a NEAT1/miR-196a-5p/GDNF regulatory pathway in CRC.
- Subjects :
- Base Sequence
Cell Line, Tumor
Cell Survival genetics
Colorectal Neoplasms genetics
Colorectal Neoplasms metabolism
Colorectal Neoplasms pathology
Glial Cell Line-Derived Neurotrophic Factor metabolism
HCT116 Cells
HT29 Cells
Humans
RNA Interference
Sequence Homology, Nucleic Acid
Cell Movement genetics
Cell Proliferation genetics
Gene Expression Regulation, Neoplastic
Glial Cell Line-Derived Neurotrophic Factor genetics
MicroRNAs genetics
RNA, Long Noncoding genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1745-7270
- Volume :
- 50
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Acta biochimica et biophysica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 30383193
- Full Text :
- https://doi.org/10.1093/abbs/gmy130