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Impacts of soil salinity on Bt protein concentration in square of transgenic Bt cotton.
- Source :
-
PloS one [PLoS One] 2018 Nov 07; Vol. 13 (11), pp. e0207013. Date of Electronic Publication: 2018 Nov 07 (Print Publication: 2018). - Publication Year :
- 2018
-
Abstract
- Insect-resistance of transgenic Bacillus thuringiensis (Bt) cotton varies among plants organs and with different environmental conditions. The objective of this study was to examine the influence of soil salinity on Bt protein concentration in cotton squares and to elucidate the potential mechanism of Bt efficacy reduction. Two cotton cultivars (NuCOTN 33B and CCRI 07, salt-sensitive and salt-tolerant) were subjected to salinity stress under four natural saline levels in field conditions in 2015 and 2016 and seven regimes of soil salinity ranged from 0.5 to 18.8 dS m-1 in greenhouse conditions in 2017. Results of field studies revealed that Bt protein content was not significantly changed at 7.13 dS m-1 salinity, but exhibited a significant drop at the 10.41 and 14.16 dS m-1 salinity. The greenhouse experiments further showed similar trends that significant declines of the insecticidal protein contents in squares were detected when soil salinity exceeded 9.1 dS m-1. Meanwhile, high salinity resulted in significant reduction in contents of soluble protein and total nitrogen, activities of nitrate reductase (NR), glutamine synthetase (GS) and glutamic-pyruvic transaminase (GPT), but increased amino acid content, activities of protease and peptidase in cotton squares. High salinity also decreased root vigor (RV), root total absorption area (RTA) and root active absorption area (RAA). The extent of decrease of Bt protein content was more pronounced in NuCOTN 33B than CCRI 07, and CCRI07 exhibited stronger enzymes activities involved in square protein synthesis and higher levels of RV, RTA and RAA. Therefore, the results of our present study indicated that insecticidal protein expression in cotton squares were significantly affected by higher salinity (equal to or higher than 9.1 dS m-1), reduced protein synthesis and increased protein degradation in squares and reduced metabolic activities in roots might lead to the decrease of Bt protein content in squares.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Alanine Transaminase metabolism
Amino Acids metabolism
Bacillus thuringiensis Toxins
Bacterial Proteins analysis
Bacterial Proteins genetics
Endotoxins analysis
Endotoxins genetics
Enzyme-Linked Immunosorbent Assay
Gossypium growth & development
Hemolysin Proteins analysis
Hemolysin Proteins genetics
Nitrate Reductase metabolism
Nitrogen analysis
Peptide Hydrolases metabolism
Plant Proteins metabolism
Plant Roots metabolism
Plants, Genetically Modified growth & development
Plants, Genetically Modified metabolism
Salinity
Salt Tolerance
Bacterial Proteins metabolism
Endotoxins metabolism
Gossypium metabolism
Hemolysin Proteins metabolism
Soil chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 13
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 30403755
- Full Text :
- https://doi.org/10.1371/journal.pone.0207013