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Discovering proteins for chemoprevention and chemotherapy by curcumin in liver fluke infection-induced bile duct cancer.
- Source :
-
PloS one [PLoS One] 2018 Nov 15; Vol. 13 (11), pp. e0207405. Date of Electronic Publication: 2018 Nov 15 (Print Publication: 2018). - Publication Year :
- 2018
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Abstract
- Modulation or prevention of protein changes during the cholangiocarcinoma (CCA) process induced by Opisthorchis viverrini (Ov) infection may become a key strategy for prevention and treatment of CCA. Monitoring of such changes could lead to discovery of protein targets for CCA treatment. Curcumin exerts anti-inflammatory and anti-CCA activities partly through its protein-modulatory ability. To support the potential use of curcumin and to discover novel target molecules for CCA treatment, we used a quantitative proteomic approach to investigate the effects of curcumin on protein changes in an Ov-induced CCA-harboring hamster model. Isobaric labelling and tandem mass spectrometry were used to compare the protein expression profiles of liver tissues from CCA hamsters with or without curcumin dietary supplementation. Among the dysregulated proteins, five were upregulated in liver tissues of CCA hamsters but markedly downregulated in the CCA hamsters supplemented with curcumin: S100A6, lumican, plastin-2, 14-3-3 zeta/delta and vimentin. Western blot and immunohistochemical analyses also showed similar expression patterns of these proteins in liver tissues of hamsters in the CCA and CCA + curcumin groups. Proteins such as clusterin and S100A10, involved in the NF-κB signaling pathway, an important signaling cascade involved in CCA genesis, were also upregulated in CCA hamsters and were then suppressed by curcumin treatment. Taken together, our results demonstrate the important changes in the proteome during the genesis of O. viverrini-induced CCA and provide an insight into the possible protein targets for prevention and treatment of this cancer.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- 14-3-3 Proteins genetics
Animals
Bile Duct Neoplasms complications
Bile Duct Neoplasms pathology
Bile Duct Neoplasms prevention & control
Chemoprevention
Cholangiocarcinoma complications
Cholangiocarcinoma genetics
Cholangiocarcinoma pathology
Cricetinae
Disease Models, Animal
Fasciola hepatica drug effects
Gene Expression Regulation drug effects
Humans
Liver drug effects
Liver pathology
Lumican genetics
Membrane Glycoproteins genetics
Microfilament Proteins genetics
Opisthorchiasis complications
Opisthorchiasis drug therapy
Opisthorchiasis genetics
Opisthorchiasis pathology
Opisthorchis pathogenicity
S100 Calcium Binding Protein A6 genetics
Vimentin genetics
Bile Duct Neoplasms drug therapy
Cholangiocarcinoma drug therapy
Curcumin administration & dosage
Proteomics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 13
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 30440021
- Full Text :
- https://doi.org/10.1371/journal.pone.0207405