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Redirecting Primary Metabolism to Boost Production of Tyrosine-Derived Specialised Metabolites in Planta.
- Source :
-
Scientific reports [Sci Rep] 2018 Nov 22; Vol. 8 (1), pp. 17256. Date of Electronic Publication: 2018 Nov 22. - Publication Year :
- 2018
-
Abstract
- L-Tyrosine-derived specialized metabolites perform many important functions in plants, and have valuable applications in human health and nutrition. A necessary step in the overproduction of specialised tyrosine-derived metabolites in planta is the manipulation of primary metabolism to enhance the availability of tyrosine. Here, we utilise a naturally occurring de-regulated isoform of the key enzyme, arogenate dehydrogenase, to re-engineer the interface of primary and specialised metabolism, to boost the production of tyrosine-derived pigments in a heterologous plant host. Through manipulation of tyrosine availability, we report a 7-fold increase in the production of tyrosine-derived betalain pigments, with an upper range of 855 mg·kg <superscript>-1</superscript> ·FW, which compare favourably to many in vitro and commercial sources of betalain pigments. Since the most common plant pathway for tyrosine synthesis occurs via arogenate, the de-regulated arogenate dehydrogenase isoform is a promising route for enhanced production of tyrosine-derived pharmaceuticals in diverse plant hosts.
- Subjects :
- Basal Metabolism
Beta vulgaris genetics
Beta vulgaris metabolism
Metabolic Engineering
Plant Proteins genetics
Plant Proteins metabolism
Prephenate Dehydrogenase genetics
Protein Isoforms metabolism
Secondary Metabolism
Nicotiana genetics
Nicotiana metabolism
Tyrosine metabolism
Beta vulgaris growth & development
Betalains metabolism
Prephenate Dehydrogenase metabolism
Nicotiana growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 30467357
- Full Text :
- https://doi.org/10.1038/s41598-018-33742-y