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Single-Cell Transcriptomics Characterizes Cell Types in the Subventricular Zone and Uncovers Molecular Defects Impairing Adult Neurogenesis.
- Source :
-
Cell reports [Cell Rep] 2018 Nov 27; Vol. 25 (9), pp. 2457-2469.e8. - Publication Year :
- 2018
-
Abstract
- Neural stem cells (NSCs) contribute to plasticity and repair of the adult brain. Niches harboring NSCs regulate stem cell self-renewal and differentiation. We used comprehensive and untargeted single-cell RNA profiling to generate a molecular cell atlas of the largest germinal region of the adult mouse brain, the subventricular zone (SVZ). We characterized >20 neural and non-neural cell types and gained insights into the dynamics of neurogenesis by predicting future cell states based on computational analysis of RNA kinetics. Furthermore, we applied our single-cell approach to document decreased numbers of NSCs, reduced proliferation activity of progenitors, and perturbations in Wnt and BMP signaling pathways in mice lacking LRP2, an endocytic receptor required for SVZ maintenance. Our data provide a valuable resource to study adult neurogenesis and a proof of principle for the power of single-cell RNA sequencing to elucidate neural cell-type-specific alterations in loss-of-function models.<br /> (Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cell Lineage
Cell Proliferation
Dentate Gyrus cytology
Gene Expression Regulation
Low Density Lipoprotein Receptor-Related Protein-2 deficiency
Low Density Lipoprotein Receptor-Related Protein-2 metabolism
Mice, Inbred C57BL
Models, Biological
Neural Stem Cells cytology
Neural Stem Cells metabolism
RNA metabolism
RNA, Long Noncoding genetics
RNA, Long Noncoding metabolism
Stem Cell Niche genetics
Aging genetics
Lateral Ventricles cytology
Neurogenesis genetics
Single-Cell Analysis
Transcriptome genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2211-1247
- Volume :
- 25
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 30485812
- Full Text :
- https://doi.org/10.1016/j.celrep.2018.11.003