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Test sensitivity of a commercial serine protease digestion kit for the detection of Trichinella spiralis and Trichinella pseudospiralis larvae in pig muscle.
- Source :
-
Food microbiology [Food Microbiol] 2019 Apr; Vol. 78, pp. 99-103. Date of Electronic Publication: 2018 Oct 22. - Publication Year :
- 2019
-
Abstract
- The reference method for Trichinella detection at meat inspection is the magnetic stirrer method (MSM) utilising HCl-pepsin for pooled sample digestion. Due to availability and quality issues with pepsin, alternative digestion methods are being offered, such as the Priocheck Trichinella AAD kit (T-AAD), based on serine endopeptidase digestion. In this study the T-AAD kit was compared to the reference method. Minced pork samples were spiked with T. spiralis muscle larvae (ML) with- and without capsule or T. pseudospiralis ML, and analysed with both tests. Test results of individually spiked test samples were analysed by generalised linear modelling. The T-AAD test kit was comparable to the reference method for the qualitative detection of T. spiralis in pigs, but not quantitatively. Overall, 94% of spiked T. spiralis were recovered using MSM against 75.2% when using T-AAD (p < 0.0001). Using the MSM 80.0% of spiked T. pseudospiralis were recovered against 20% with the T-AAD (p < 0.0001). Based on our experience with the T-AAD kit, we strongly recommend validating the method on site prior to introduction into routine diagnostic laboratories, but this will not alleviate the poor test sensitivity of the T-AAD for the detection of T. pseudospiralis.<br /> (Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Subjects :
- Animals
Food Inspection
Magnetic Fields
Male
Reagent Kits, Diagnostic
Sensitivity and Specificity
Serine Endopeptidases metabolism
Swine
Swine Diseases parasitology
Trichinellosis diagnosis
Trichinellosis parasitology
Trichinellosis veterinary
Larva physiology
Meat parasitology
Muscles parasitology
Serine Proteases metabolism
Swine Diseases diagnosis
Trichinella isolation & purification
Trichinella spiralis isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9998
- Volume :
- 78
- Database :
- MEDLINE
- Journal :
- Food microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 30497613
- Full Text :
- https://doi.org/10.1016/j.fm.2018.10.012