Mutations in cdsA and pgsA Correlate with Daptomycin Resistance in Streptococcus mitis and S. oralis .

Authors :: Tran TT
Mishra NN
Seepersaud R
Diaz L
Rios R
Dinh AQ
Garcia-de-la-Maria C
Rybak MJ
Miro JM
Shelburne SA
Sullam PM
Bayer AS
Arias CA
Source :: Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2019 Jan 29; Vol. 63 (2). Date of Electronic Publication: 2019 Jan 29 (Print Publication: 2019).
Publication Year :: 2019
Abstract: We investigated the ability of several recent clinical viridans group streptococci (VGS) bloodstream isolates ( Streptococcus mitis / S. oralis subgroup) from daptomycin (DAP)-naive patients to develop DAP resistance in vitro All strains rapidly developed high-level and stable DAP resistance. Substitutions in two enzymes involved in the cardiolipin biosynthesis pathway were identified, i.e., CdsA (phosphatidate cytidylyltransferase) and PgsA (CDP-diacylglycerol-glycerol-3-phosphate-3-phosphatidyltransferase). These mutations were associated with complete disappearance of phosphatidylglycerol and cardiolipin from cell membranes. DAP interactions with the cell membrane differed in isolates with PgsA versus CdsA substitutions.<br /> (Copyright © 2019 American Society for Microbiology.)

Subjects :: Cardiolipins metabolism
Cell Membrane genetics
Cell Membrane metabolism
Drug Resistance, Bacterial genetics
Humans
Microbial Sensitivity Tests
Phosphatidylglycerols metabolism
Streptococcus mitis drug effects
Streptococcus mitis isolation & purification
Streptococcus oralis drug effects
Streptococcus oralis isolation & purification
Anti-Bacterial Agents pharmacology
Daptomycin pharmacology
Nucleotidyltransferases genetics
Streptococcus mitis genetics
Streptococcus oralis genetics
Transferases (Other Substituted Phosphate Groups) genetics

Full Text Access

Tools