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Histone deacetylase inhibitors suppress transdifferentiation of gonadotrophs to prolactin cells and proliferation of prolactin cells induced by diethylstilbestrol in male mouse pituitary.
- Source :
-
Histochemistry and cell biology [Histochem Cell Biol] 2019 Apr; Vol. 151 (4), pp. 291-303. Date of Electronic Publication: 2018 Dec 03. - Publication Year :
- 2019
-
Abstract
- Diethylstilbestrol (DES), an estrogen agonist, increases prolactin (PRL) cells through transdifferentiation of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) cells to PRL cells as well as proliferation of PRL cells in adult male mouse pituitary. Since hyperacetylation of histone H3 is implicated in the regulation of activation of various genes, we examined the effect of DES on the state of histone H3 acetylation. DES significantly reduced the immunohistochemical signal for acetylated histone H3 at lysine 9 (H3K9ac) in PRL, LH and FSH cells, but not for H3K18ac or H3K23ac. DES-treated mice were injected intraperitoneally with HDAC inhibitors (HDACi), sodium phenylbutyrate (NaPB) or valproic acid (VPA), to mimic the acetylation level of histone H3. As expected, HDACi treatment restored the level of H3K9ac expression in these cells, and also inhibited DES-induced increase in PRL cells. Furthermore, NaPB and VPA also abrogated the effects of DES on the population density of both LH and FSH cells. Similarly, the numbers of proliferating and apoptotic cells in the pituitary in NaPB- or VPA-treated mice were comparable to those of the control mice. Considered together, these results indicated that the acetylation level of histone H3 plays an important role in DES-induced transdifferentiation of LH to PRL cells as well as proliferation of PRL cells.
- Subjects :
- Acetylation drug effects
Animals
Apoptosis drug effects
Cell Proliferation drug effects
Diethylstilbestrol administration & dosage
Diethylstilbestrol pharmacology
Gonadotrophs cytology
Histone Deacetylase Inhibitors administration & dosage
Histones analysis
Histones biosynthesis
Injections, Intraperitoneal
Lactotrophs cytology
Male
Mice
Mice, Inbred ICR
Phenylbutyrates administration & dosage
Pituitary Gland metabolism
Rabbits
Valproic Acid administration & dosage
Cell Transdifferentiation drug effects
Gonadotrophs drug effects
Histone Deacetylase Inhibitors pharmacology
Lactotrophs drug effects
Phenylbutyrates pharmacology
Pituitary Gland drug effects
Valproic Acid pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1432-119X
- Volume :
- 151
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Histochemistry and cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 30511269
- Full Text :
- https://doi.org/10.1007/s00418-018-1760-z