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[Anti-scarring effect of rapamycin in rabbits following glaucoma filtering surgery].
- Source :
-
Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2018 Nov 30; Vol. 38 (11), pp. 1389-1394. - Publication Year :
- 2018
-
Abstract
- Objective: To study the anti- scarring effect of rapamycin in rabbits receiving glaucoma filtering surgery.<br />Methods: Ninety-six Chinchilla rabbits were randomized equally into 3 rapamycin treatment groups and one control group. All the rabbits underwent trabeculectomy, after which the rabbits in the 3 rapamycin groups were treated with eye drops containing 1%, 3%, or 5% rapamycin in the operated eyes, and those in the control groups were given castor oil 4 times a day. The intraocular pressure (IOP) and inflammatory reaction in the treated eyes were observed, and the PCNA-positive cells in the filtering bleb were detected using immunohistochemistry. RTFs isolated from the Tenon's capsule of the rabbits were cultured in vitro , and the expressions of caspase-3, caspase-8, and caspase-9 in the fibroblasts were detected after treatment with different concentrations of rapamycin.<br />Results: The IOP was significantly lower in rapamycin-treated group than in the control group after the surgery ( P &lt; 0.05). The counts of the PCNA-positive cells were significantly lower in rapamycin-treated rabbits than in the control group ( P &lt; 0.05). Rapamycin treatment dose-dependently increased the expressions of caspase-3 and caspase- 9 at both the mRNA ( P &lt; 0.001) and protein ( P &lt; 0.001) levels without causing significant changes in the expressions of caspase-8.<br />Conclusions: Rapamycin can inhibit excessive proliferation of the fibroblasts in the filtering bleb to reduce scar formation after glaucoma filtration surgery in rabbits. Rapamycin also increases the expressions of caspase-3 and caspase-9 to induce apoptosis of the RTFs.
- Subjects :
- Animals
Caspase 3 metabolism
Caspase 9 metabolism
Cell Proliferation drug effects
Intraocular Pressure
Postoperative Complications enzymology
Proliferating Cell Nuclear Antigen analysis
Rabbits
Random Allocation
Trabeculectomy
Cicatrix prevention & control
Filtering Surgery adverse effects
Glaucoma surgery
Postoperative Complications prevention & control
Sirolimus therapeutic use
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1673-4254
- Volume :
- 38
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University
- Publication Type :
- Academic Journal
- Accession number :
- 30514691
- Full Text :
- https://doi.org/10.12122/j.issn.1673-4254.2018.11.19