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Head and neck cancer organoids established by modification of the CTOS method can be used to predict in vivo drug sensitivity.
- Source :
-
Oral oncology [Oral Oncol] 2018 Dec; Vol. 87, pp. 49-57. Date of Electronic Publication: 2018 Oct 23. - Publication Year :
- 2018
-
Abstract
- Objectives: Currently there are no standard biomarkers of head and neck squamous cell carcinoma (HNSCC) response to therapy. This is, due to a lack of adequate predictive tumor models. To this end, we established cancer organoid lines from individual patient's tumors, and characterized their growth characteristics and response to different drug treatments with the objective of using these models for prediction of treatment response.<br />Materials and Methods: Forty-three patients' samples were processed to establish organoids. To analyze the character of these organoids, immunohistochemistry, Western blotting, drug sensitivity assays, clonogenic survival assays, and animal experiments were performed. The HPV status and TP53 mutational status were also confirmed in these lines.<br />Results: HNSCC organoids were successfully established with success rate of 30.2%. Corresponding two-dimensional cell lines were established from HNSCC organoids at higher success rate (53.8%). These organoids showed similar histological features and stem cell, epithelial and mesenchymal marker expression to the original tumors, thus recapitulating many of the characteristics of the original tumor cells. The cisplatin and docetaxel IC50 were determined for HNSCC organoids and the corresponding 2D cell lines using drug sensitivity and clonogenic survival assays. Responses to drug treatment in vivo were found to be similar to the IC <subscript>50</subscript> calculated from organoids by drug sensitivity assays in vitro.<br />Conclusion: We established novel in vitro HNSCC cancer organoid lines retaining many properties of the original tumors from they were derived. These organoids can predict in vivo drug sensitivity and may represent useful tools to develop precision treatments for HNSCC.<br /> (Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Subjects :
- Animals
Antineoplastic Agents therapeutic use
Cisplatin pharmacology
Cisplatin therapeutic use
Docetaxel pharmacology
Docetaxel therapeutic use
Drug Screening Assays, Antitumor methods
Female
Head and Neck Neoplasms pathology
Humans
Inhibitory Concentration 50
Male
Mice
Organoids pathology
Spheroids, Cellular drug effects
Spheroids, Cellular pathology
Squamous Cell Carcinoma of Head and Neck pathology
Tumor Cells, Cultured
Xenograft Model Antitumor Assays
Antineoplastic Agents pharmacology
Head and Neck Neoplasms drug therapy
Organoids drug effects
Primary Cell Culture methods
Squamous Cell Carcinoma of Head and Neck drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0593
- Volume :
- 87
- Database :
- MEDLINE
- Journal :
- Oral oncology
- Publication Type :
- Academic Journal
- Accession number :
- 30527243
- Full Text :
- https://doi.org/10.1016/j.oraloncology.2018.10.018