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Fingerprints of CD8+ T cells on human pre-plasma and memory B cells.
- Source :
-
PloS one [PLoS One] 2018 Dec 12; Vol. 13 (12), pp. e0208187. Date of Electronic Publication: 2018 Dec 12 (Print Publication: 2018). - Publication Year :
- 2018
-
Abstract
- Differentiation of B cells is a stringently controlled multi-step process, which is still incompletely understood. Here we identify and characterize a rare population of human B cells, which surprisingly carry CD8AB on their surface. Existence of such cells was demonstrated both in tonsils and in human apheresis material. Gene expression profiling and real time PCR detected however no CD8A or CD8B message in these cells. Instead, we found that surface CD8 was hijacked from activated CD8+ T cells by a transfer process that required direct cell-to-cell contact. A focused transcriptome analysis at single cell level allowed the dissection of the CD8 positive B cell population. We found that the affected cells are characteristically of the CD27+CD200- phenotype, and consist of two discrete late-stage subpopulations that carry signatures of activated memory B like cells, and early plasmablasts. Thus, there is only a restricted time window in the differentiation process during which B cells can intimately interact with CD8+ T cells. The findings point to a novel link between the T and B arms of the adaptive immune system, and suggest that CD8+ T cells have the capability to directly shape the global antibody repertoire.<br />Competing Interests: The authors are employees of Novartis and declare no other competing interest. The commercial affiliation does not alter the authors' adherence to PLOS ONE policies on sharing data and materials.
- Subjects :
- Antigens, CD genetics
Antigens, CD metabolism
B-Lymphocyte Subsets metabolism
CD8 Antigens genetics
CD8 Antigens immunology
CD8-Positive T-Lymphocytes metabolism
Cell Differentiation immunology
Cell Separation
Cells, Cultured
Flow Cytometry
Gene Expression Profiling
Healthy Volunteers
Humans
Primary Cell Culture
RNA, Messenger analysis
Single-Cell Analysis
Tumor Necrosis Factor Receptor Superfamily, Member 7 genetics
Tumor Necrosis Factor Receptor Superfamily, Member 7 metabolism
B-Lymphocyte Subsets immunology
CD8 Antigens metabolism
CD8-Positive T-Lymphocytes microbiology
Cell Communication immunology
Immunologic Memory
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 13
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 30540814
- Full Text :
- https://doi.org/10.1371/journal.pone.0208187