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LncRNA-H19 activates CDC42/PAK1 pathway to promote cell proliferation, migration and invasion by targeting miR-15b in hepatocellular carcinoma.
- Source :
-
Genomics [Genomics] 2019 Dec; Vol. 111 (6), pp. 1862-1872. Date of Electronic Publication: 2018 Dec 10. - Publication Year :
- 2019
-
Abstract
- Background: Hepatocellular carcinoma (HCC) is one of the main causes of cancer-related death. This study aims to explore the role and underlying mechanism of H19 in HCC.<br />Methods: qRT-PCR detected miR-15b-5p and H19 expression, as well as the mRNA level of EMT-associated genes. Western blotting detected protein level of EMT-associated genes. Immunohistochemistry (IHC) examined CDC42 in HCC tissues. Dual luciferase reporter assay verified the regulatory mechanism among H19, miR-15b and CDC42. Colony formation, wound healing assay, transwell, flow cytometry measured proliferation, migration, invasion and apoptosis, respectively.<br />Results: H19 and CDC42 were up-regulated while miR-15b was down-regulated in HCC cells and tissues. miR-15b interacted with H19 and CDC42 3'-UTR. H19 knockdown inhibited proliferation, migration and invasion, and increased apoptosis, which was rescued by miR-15b inhibitor. H19 knockdown suppressed CDC42/PAK1 pathway and EMT progress.<br />Conclusion: H19 knockdown inhibited proliferation, migration and invasion, and promoted apoptosis of HCC cells via targeting miR-15b/CDC42/PAK1 axis.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Subjects :
- Adult
Aged
Female
Hep G2 Cells
Humans
Male
Middle Aged
Neoplasm Invasiveness
Carcinoma, Hepatocellular genetics
Carcinoma, Hepatocellular metabolism
Carcinoma, Hepatocellular pathology
Cell Movement genetics
Cell Proliferation genetics
Liver Neoplasms genetics
Liver Neoplasms metabolism
Liver Neoplasms pathology
MicroRNAs genetics
MicroRNAs metabolism
Neoplasm Proteins genetics
Neoplasm Proteins metabolism
RNA, Long Noncoding genetics
RNA, Long Noncoding metabolism
RNA, Neoplasm genetics
RNA, Neoplasm metabolism
cdc42 GTP-Binding Protein genetics
cdc42 GTP-Binding Protein metabolism
p21-Activated Kinases genetics
p21-Activated Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1089-8646
- Volume :
- 111
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Genomics
- Publication Type :
- Academic Journal
- Accession number :
- 30543848
- Full Text :
- https://doi.org/10.1016/j.ygeno.2018.12.009