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A genome-wide dsRNA library screen for Drosophila genes that regulate the GBP/phospholipase C signaling axis that links inflammation to aging.
- Source :
-
BMC research notes [BMC Res Notes] 2018 Dec 13; Vol. 11 (1), pp. 884. Date of Electronic Publication: 2018 Dec 13. - Publication Year :
- 2018
-
Abstract
- Objective: Invertebrates are productive models for understanding how inflammation, metabolism and aging are intertwined. We have deployed a dsRNA library screen to search for genes in Drosophila melanogaster-and hence identify human orthologs-that encode participants in a G-protein coupled, Ca <superscript>2+</superscript> -signaling pathway that regulates inflammation, metabolism and lifespan.<br />Results: We analyzed receptor-dependent, phospholipase C/Ca <superscript>2+</superscript> signaling responses to the growth-blocking peptide (GBP) cytokine in Drosophila S3 cells plated in 384-well plates containing dsRNAs that target approximately 14,000 Drosophila genes. We used Z-scores of < - 3 or > + 3 to define gene hits. Filtering of 'housekeeping' genes from these hits yielded a total of 82 and 61 Drosophila genes that either down-regulate or up-regulate Ca <superscript>2+</superscript> -signaling, respectively; representatives from these two groups were validated. Human orthologs of our hits may be modulators of Ca <superscript>2+</superscript> signaling in general, as well as being candidates for acting in molecular pathways that interconnect aging and inflammation.
- Subjects :
- Animals
Calcium Signaling genetics
Cytokines genetics
Drosophila Proteins metabolism
Gene Knockdown Techniques
Genetic Testing
Reproducibility of Results
Type C Phospholipases genetics
Aging genetics
Drosophila Proteins genetics
Drosophila melanogaster genetics
Genes, Insect
Genomic Library
Inflammation genetics
RNA, Double-Stranded genetics
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 1756-0500
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC research notes
- Publication Type :
- Academic Journal
- Accession number :
- 30545410
- Full Text :
- https://doi.org/10.1186/s13104-018-3996-z