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Characterization of regulatory T cells in obese omental adipose tissue in humans.
- Source :
-
European journal of immunology [Eur J Immunol] 2019 Feb; Vol. 49 (2), pp. 336-347. Date of Electronic Publication: 2019 Jan 08. - Publication Year :
- 2019
-
Abstract
- Obesity-associated visceral adipose tissue (AT) inflammation promotes insulin resistance and type 2 diabetes (T2D). In mice, lean visceral AT is populated with anti-inflammatory cells, notably regulatory T cells (Tregs) expressing the IL-33 receptor ST2. Conversely, obese AT contains fewer Tregs and more proinflammatory cells. In humans, however, there is limited evidence for a similar pattern of obesity-associated immunomodulation. We used flow cytometry and mRNA quantification to characterize human omental AT in 29 obese subjects, 18 of whom had T2D. Patients with T2D had increased proportions of inflammatory cells, including M1 macrophages, with positive correlations to body mass index. In contrast, Treg frequencies negatively correlated to body mass index but were comparable between T2D and non-T2D individuals. Compared to human thymic Tregs, omental AT Tregs expressed similar levels of FOXP3, CD25, IKZF2, and CTLA4, but higher levels of PPARG, CCR4, PRDM1, and CXCL2. ST2, however, was not detectable on omental AT Tregs from lean or obese subjects. This is the first comprehensive investigation into how omental AT immunity changes with obesity and T2D in humans, revealing important similarities and differences to paradigms in mice. These data increase our understanding of how pathways of immune regulation could be targeted to ameliorate AT inflammation in humans.<br /> (© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Adipose Tissue pathology
Adult
Antigens, Differentiation immunology
Diabetes Mellitus, Type 2 pathology
Female
Humans
Inflammation immunology
Inflammation pathology
Male
Obesity pathology
Panniculitis pathology
T-Lymphocytes, Regulatory pathology
Adipose Tissue immunology
Diabetes Mellitus, Type 2 immunology
Obesity immunology
Panniculitis immunology
T-Lymphocytes, Regulatory immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1521-4141
- Volume :
- 49
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- European journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 30566246
- Full Text :
- https://doi.org/10.1002/eji.201847570