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Paper Electrode-Based Flexible Pressure Sensor for Point-of-Care Immunoassay with Digital Multimeter.
- Source :
-
Analytical chemistry [Anal Chem] 2019 Jan 15; Vol. 91 (2), pp. 1222-1226. Date of Electronic Publication: 2018 Dec 21. - Publication Year :
- 2019
-
Abstract
- A novel paper electrode-based flexible pressure sensor modified with multiwalled carbon nanotubes was designed for point-of-care (POC) immunoassay of carcinoembryonic antigen (CEA) with digital multimeter readout. The portable POC testing device consisted of flexible pressure sensor equipped with a paper electrode and connected through syringe tubing to a single-break microplate. The immunoreaction was initially carried out on the microplate with a sandwich-type assay format using platinum nanozyme-labeled secondary antibody for the gas generation. Upon addition of hydrogen peroxide (H <subscript>2</subscript> O <subscript>2</subscript> ), platinum nanozyme (catalase-like mimic) reduced it into hydrogen oxide and oxygen (O <subscript>2</subscript> ). The overflowing oxygen gas increased the pressure of the multiwalled carbon nanotube-functionalized paper electrode in a homemade pressure-tight system, and the increased pressure could be readily monitored using the paper electrode-based flexible pressure-tight sensor with a digital multimeter readout. The detectable signal mainly derived from the resistance change of pressure sensor because of its deformation with the assistance of the as-generated gas, and the shift in the resistance could be allowed to detect the gas pressure even as low as 80 Pa. Under optimum conditions, pressure sensor-based immunoassay exhibited good resistance responses toward target CEA within a linear range of 0.5-60 ng/mL at a detection limit of 167 pg/mL. Moreover, our strategy provided acceptable reproducibility, precision, high specificity, and good accordance with the commercial CEA ELISA kit for detecting human serum specimens.
- Subjects :
- Antibodies, Immobilized immunology
Biomarkers blood
Carcinoembryonic Antigen immunology
Catalysis
Electrochemical Techniques instrumentation
Electrochemical Techniques methods
Humans
Hydrogen Peroxide chemistry
Limit of Detection
Metal Nanoparticles chemistry
Nanotubes, Carbon chemistry
Platinum
Point-of-Care Testing
Pressure
Carcinoembryonic Antigen blood
Electrodes
Immunoassay methods
Oxygen chemistry
Paper
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6882
- Volume :
- 91
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 30569701
- Full Text :
- https://doi.org/10.1021/acs.analchem.8b04635