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Ultralong TE In Vivo 1 H MR Spectroscopy of Omega-3 Fatty Acids in Subcutaneous Adipose Tissue at 7 T.
- Source :
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Journal of magnetic resonance imaging : JMRI [J Magn Reson Imaging] 2019 Jul; Vol. 50 (1), pp. 71-82. Date of Electronic Publication: 2018 Dec 21. - Publication Year :
- 2019
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Abstract
- Background: Omega-3 (n-3) fatty acids (FA) play and important role in neural development and other metabolic diseases such as obesity and diabetes. The knowledge about the in vivo content and distribution of n-3 FA in human body tissues is not well established and the standard quantification of FA is invasive and costly.<br />Purpose: To detect omega-3 (n-3 CH <subscript>3</subscript> ) and non-omega-3 (CH <subscript>3</subscript> ) methyl group resonance lines with echo times up to 1200 msec, in oils, for the assessment of n-3 FA content, and the n-3 FA fraction in adipose tissue in vivo.<br />Study Type: Prospective technical development.<br />Population: Three oils with different n-3 FA content and 24 healthy subjects.<br />Field Strength/sequence: Single-voxel MR spectroscopy (SVS) with a point-resolved spectroscopy (PRESS) sequence with an echo time (TE) of 1000 msec at 7 T.<br />Assessment: Knowledge about the J-coupling evolution of both CH <subscript>3</subscript> resonances was used for the optimal detection of the n-3 CH <subscript>3</subscript> resonance line at a TE of 1000 msec. The accuracy of the method in oils and in vivo was validated from a biopsy sample with gas chromatography analysis.<br />Statistical Tests: SVS data were compared to gas chromatography with the Pearson correlation coefficient.<br />Results: T <subscript>2</subscript> relaxation times in oils were assessed as follows: CH <subscript>2</subscript> , 65 ± 22 msec; CH <subscript>3</subscript> , 325 ± 7 msec; and n-3 CH <subscript>3</subscript> , 628 ± 34 msec. The n-3 FA fractions from oil phantom experiments (n = 3) were in agreement with chromatography analysis and the comparison of in vivo obtained data with the results of chromatography analysis (n = 5) yielded a significant correlation (P = 0.029).<br />Data Conclusion: PRESS with ultralong-TE can detect and quantify the n-3 CH <subscript>3</subscript> signal in vivo at 7 T.<br />Level of Evidence: 1 Technical Efficacy: Stage 1 J. Magn. Reson. Imaging 2019;50:71-82.<br /> (© 2018 The Authors. Journal of Magnetic Resonance Imaging published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine.)
Details
- Language :
- English
- ISSN :
- 1522-2586
- Volume :
- 50
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of magnetic resonance imaging : JMRI
- Publication Type :
- Academic Journal
- Accession number :
- 30578581
- Full Text :
- https://doi.org/10.1002/jmri.26605