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Viperin Inhibits Enterovirus A71 Replication by Interacting with Viral 2C Protein.
- Source :
-
Viruses [Viruses] 2018 Dec 26; Vol. 11 (1). Date of Electronic Publication: 2018 Dec 26. - Publication Year :
- 2018
-
Abstract
- Enterovirus A71 (EVA71) is a human enterovirus belonging to the Picornaviridae family and mostly causes hand-foot-and-mouth disease in infants. Viperin is an important interferon-stimulated gene with a broad antiviral activity against various viruses. However, the effect of viperin on human enteroviruses and the interaction mechanism between EVA71 and viperin remains elusive. Here, we confirmed the EVA71-induced expression of viperin in a mouse model and cell lines and showed that viperin upregulation by EVA71 infection occurred on both the mRNA and protein level. Viperin knockdown and overexpression in EVA71-infected cells indicated that this protein can markedly inhibit EVA71 infection. Interestingly, immunofluorescent confocal microscopy and co-immunoprecipitation assays indicated that viperin interacts and colocalizes with the EVA71 protein 2C in the endoplasmic reticulum. Furthermore, amino acids 50⁻60 in the N-terminal domain of viperin were the key residues responsible for viperin interaction with 2C. More importantly, the N-terminal domain of viperin was found responsible for inhibiting EVA71 replication. Our findings can potentially aid future research on the prevention and treatment of nervous system damage caused by EVA71 and may provide a potential target for antiviral therapy.
- Subjects :
- Animals
Cell Line, Tumor
Endoplasmic Reticulum chemistry
Endoplasmic Reticulum virology
Enterovirus Infections virology
Fluorescent Antibody Technique
Gene Knockdown Techniques
HEK293 Cells
Humans
Mice
Mice, Inbred BALB C
Microscopy, Confocal
Oxidoreductases Acting on CH-CH Group Donors
Proteins metabolism
RNA, Messenger
Up-Regulation
Carrier Proteins metabolism
Enterovirus physiology
Host Microbial Interactions
Proteins genetics
Viral Nonstructural Proteins metabolism
Virus Replication
Subjects
Details
- Language :
- English
- ISSN :
- 1999-4915
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Viruses
- Publication Type :
- Academic Journal
- Accession number :
- 30587778
- Full Text :
- https://doi.org/10.3390/v11010013